CAJ | 학술논문

为了建立天山花楸叶中总黄酮苷含量的测定方法,对样品酸水解处理再进行HPLC分析,色谱柱为Waters-C18（150 mm×4.6 mm,5μm）,柱温25℃,使用可变波长检测器;流动相为甲醇-乙腈-0.4%磷酸水溶液,梯度洗脱程序为0 min（12∶20∶68）-10 min（52∶0∶48）-18 min（72∶0∶28）;流速1.0 mL/min,检测波长为360 nm。结果表明：槲皮素浓度在5.98～47.84μg/mL之间与色谱峰面积呈良好的线性关系,回归方程A=66.96C-5.9964,r=0.9995。平均回收率94.86%,RSD=1.9%;山柰素浓度在4.04～28.28μg/mL之间与色谱峰面积呈良好的线性关系,回归方程A=50.114C-19.529,r=0.9999,平均回收率93.89%,RSD=2.7%;其黄酮苷百分含量为（12.62±0.40）%。该方法干扰少,灵敏,简便,重现性好,可为天山花楸叶的药用开发利用提供质量控制方法。
위료건립천산화추협중총황동감함량적측정방법,대양품산수해처리재진행HPLC분석,색보주위Waters-C18（150 mm×4.6 mm,5μm）,주온25℃,사용가변파장검측기;류동상위갑순-을정-0.4%린산수용액,제도세탈정서위0 min（12∶20∶68）-10 min（52∶0∶48）-18 min（72∶0∶28）;류속1.0 mL/min,검측파장위360 nm。결과표명：곡피소농도재5.98～47.84μg/mL지간여색보봉면적정량호적선성관계,회귀방정A=66.96C-5.9964,r=0.9995。평균회수솔94.86%,RSD=1.9%;산내소농도재4.04～28.28μg/mL지간여색보봉면적정량호적선성관계,회귀방정A=50.114C-19.529,r=0.9999,평균회수솔93.89%,RSD=2.7%;기황동감백분함량위（12.62±0.40）%。해방법간우소,령민,간편,중현성호,가위천산화추협적약용개발이용제공질량공제방법。
An method for determination of the content of flavonoid glycosides extracted from Sorbus tianschnica was established.The samples,which were hydrolyzed with acid,were analyzed by RP-HPLC.The analysis was carried out on a waters-C18（4.6 mm×150 mm,5 μm） column,The mobile phase was composed of A（methanol）-phase B（acetonitrile）-phase C（0.4% phosphoric acid water solution） in gradient elution 0 min（12：20：68）-10 min（52：0：48）-18 min（72：0：28） by flow rate of 1.0 mL/min in this method,the detection wave length was set at 360 nm,the VWD was used and the column temperature was 25 ℃.The linear relationship of quercetin was good 5.98~47.84 μg/mL,A=66.96C-5.9964,and r=0.9995.The average recovery was 94.86%,（RSD=1.9%）,the linear relationship of kaempferol was good 4.04~28.28 μg/mL,A=50.114C-19.529,and r=0.9999.The average recovery was 93.89%,（RSD=2.7%）.The content of flavonoid glycosides was（12.62±0.40）%.This method was simple,sensitive and less interference,reproducible,the utilization of quality control method with the leaf of Sorbus tianschanica.