广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2012年
15期
150-152
,共3页
高效液相色谱法%单硝酸异山梨酯%血药浓度
高效液相色譜法%單硝痠異山梨酯%血藥濃度
고효액상색보법%단초산이산리지%혈약농도
high performance liquid chromatography%isosorbide-5-mononitrate%plasma drug concentration
建立了动物血浆单硝酸异山梨酯检测的高效液相色谱方法。血浆经高速离心后,以Beckman ODS为色谱柱,流动相为乙腈-水（10∶90,v/v）,流速为1.0 mL/min,柱温为35℃,检测波长为220 nm。并用此方法测定4只家兔单剂量口服40 mg单硝酸异山梨酯片后的血药浓度经时过程。结果显示：所建立的测定单硝酸异山梨酯血药浓度HPLC方法在5～500 ng/mL范围内线性关系良好,定量下限为5 ng/mL;高、中、低3个浓度的方法回收率在92%～107%之间,日内精密度和日间精密度约在6%以内。结论本方法简便、快速、准确、可靠,适用于动物血浆中单硝酸异山梨酯浓度的测定。
建立瞭動物血漿單硝痠異山梨酯檢測的高效液相色譜方法。血漿經高速離心後,以Beckman ODS為色譜柱,流動相為乙腈-水（10∶90,v/v）,流速為1.0 mL/min,柱溫為35℃,檢測波長為220 nm。併用此方法測定4隻傢兔單劑量口服40 mg單硝痠異山梨酯片後的血藥濃度經時過程。結果顯示：所建立的測定單硝痠異山梨酯血藥濃度HPLC方法在5～500 ng/mL範圍內線性關繫良好,定量下限為5 ng/mL;高、中、低3箇濃度的方法迴收率在92%～107%之間,日內精密度和日間精密度約在6%以內。結論本方法簡便、快速、準確、可靠,適用于動物血漿中單硝痠異山梨酯濃度的測定。
건립료동물혈장단초산이산리지검측적고효액상색보방법。혈장경고속리심후,이Beckman ODS위색보주,류동상위을정-수（10∶90,v/v）,류속위1.0 mL/min,주온위35℃,검측파장위220 nm。병용차방법측정4지가토단제량구복40 mg단초산이산리지편후적혈약농도경시과정。결과현시：소건립적측정단초산이산리지혈약농도HPLC방법재5～500 ng/mL범위내선성관계량호,정량하한위5 ng/mL;고、중、저3개농도적방법회수솔재92%～107%지간,일내정밀도화일간정밀도약재6%이내。결론본방법간편、쾌속、준학、가고,괄용우동물혈장중단초산이산리지농도적측정。
HPLC method was developed for assay of isosorbide mononitrate（IM） blood level in rabbit.After the centrifugation of blood,the reconstituted samples were analyzed on Beckman ODS column at the wavelength of 220 nm using a mobile phase consisting of acetonitrile-d.d.H2O（10：90,v/v） at a flow rate of 1.0 mL/min and column oven at 35 ℃.IM blood levels in the rabbits exposed to 40-mg IM tablet were assayed.There existed linear relationship between the peak areas in the HPLC system and the concentrations of IM in the spiked samples with the low detection limit of 5 ng/ml.The method recoveries of 92%~107% were found when the samples of high,middle and low levels of interest were loaded.Moreover,the inter-day and intra-day variation coefficients were estimated in 6%.The method was simple,rapid,accurate,and reliable,which was suitable for the determination of IM blood level in rabbit.
