CAJ | 학술논문

以自然海水为基础溶液,用二甲亚砜、乙二醇、甘油分别配制不同体积分数的抗冻保护剂溶液,用以激活虾夷马粪海胆精子并且观察其精子活力、受精率及其形态学上的改变。结果表明,经4%~18%的抗冻保护剂处理过的样本精子皆可被激活,并具有受精能力,若抗冻保护剂浓度达到18%以上,则精子全部失活。经4%乙二醇处理过的样本精子活力为最高,平均可达到76.44%（P≤0.05）,但在各浓度梯度的样本中均以二甲亚砜处理过的样本受精率最高。这3种抗冻保护剂均对虾夷马粪海胆精子有毒害作用,并且对其精子的活力和受精率有一定的影响。
이자연해수위기출용액,용이갑아풍、을이순、감유분별배제불동체적분수적항동보호제용액,용이격활하이마분해담정자병차관찰기정자활력、수정솔급기형태학상적개변。결과표명,경4%~18%적항동보호제처리과적양본정자개가피격활,병구유수정능력,약항동보호제농도체도18%이상,칙정자전부실활。경4%을이순처리과적양본정자활력위최고,평균가체도76.44%（P≤0.05）,단재각농도제도적양본중균이이갑아풍처리과적양본수정솔최고。저3충항동보호제균대하이마분해담정자유독해작용,병차대기정자적활력화수정솔유일정적영향。
Using natural sea water as the basic menstruum,the cryoprotectant solutions were prepared by dimethyl-sulfoxide, ethylene glycol and glycerol with different volume fractions respectively. The Strongylocentrtus intermedius sperm was activated by utilizing the solution and observe its motility, fertilization rate as well as its morphological changes. The experimental results demonstrated that all of sperm treated by cryoprotectant solution of concentration level 4%-18% could be activated and possessed the fertilization ability as well. The cryoproteetant samples with concentration level above 18% had toxicity which could deactivate all the sperm ,while the sperm motility of the samples treated by 4% ethylene glycol was the highest, which could reach the average level of 76.44% （P ≤ 0.05 ）. However, the fertilization rate of the samples treated by dimethyl-sulfoxide was the highest in each concentration level. These three cryoprotectants were all poisonous to Strongylocentrotus intermedius sperm,and the sperm motility and fertilization rate both declined after being treated.