龙岩学院学报
龍巖學院學報
룡암학원학보
JOURNAL OF LONGYAN UNIVERSITY
2012年
5期
76-82
,共7页
桑庆亮%陈春玲%黄周英%陈怀宇%罗丽娟%蔡英卿%陈朝阳
桑慶亮%陳春玲%黃週英%陳懷宇%囉麗娟%蔡英卿%陳朝暘
상경량%진춘령%황주영%진부우%라려연%채영경%진조양
花叶玉簪%组织培养%快速繁殖%试管苗
花葉玉簪%組織培養%快速繁殖%試管苗
화협옥잠%조직배양%쾌속번식%시관묘
Hosta plantaginea%tissue culture%rapid proliferation%plantlets
利用花叶玉簪试管苗进行其快速繁殖、壮苗生根与移栽实验,结果表明,在MS基本培养基中附加低浓度6-BA（0.1～0.5 mg/L）和生长素（IBA 0.01 mg/L或NAA 0.1 mg/L）有利于花叶玉簪试管苗离体分株;而附加高浓度6-BA（≥1.0 mg/L）和生长素（IBA 0.01 mg/L或NAA0.1 mg/L）则有利于花叶玉簪愈伤组织分化产生不定芽。MS培养基附加低浓度6-BA（0.1～0.5mg/L）或不加6-BA,配合一定浓度的生长素（IBA 0.1～0.3 mg/L或NAA 0.1 mg/L）有利于花叶玉簪试管苗壮苗生根。生根良好的花叶玉簪试管苗移栽成活率达91%±2%。
利用花葉玉簪試管苗進行其快速繁殖、壯苗生根與移栽實驗,結果錶明,在MS基本培養基中附加低濃度6-BA（0.1～0.5 mg/L）和生長素（IBA 0.01 mg/L或NAA 0.1 mg/L）有利于花葉玉簪試管苗離體分株;而附加高濃度6-BA（≥1.0 mg/L）和生長素（IBA 0.01 mg/L或NAA0.1 mg/L）則有利于花葉玉簪愈傷組織分化產生不定芽。MS培養基附加低濃度6-BA（0.1～0.5mg/L）或不加6-BA,配閤一定濃度的生長素（IBA 0.1～0.3 mg/L或NAA 0.1 mg/L）有利于花葉玉簪試管苗壯苗生根。生根良好的花葉玉簪試管苗移栽成活率達91%±2%。
이용화협옥잠시관묘진행기쾌속번식、장묘생근여이재실험,결과표명,재MS기본배양기중부가저농도6-BA（0.1～0.5 mg/L）화생장소（IBA 0.01 mg/L혹NAA 0.1 mg/L）유리우화협옥잠시관묘리체분주;이부가고농도6-BA（≥1.0 mg/L）화생장소（IBA 0.01 mg/L혹NAA0.1 mg/L）칙유리우화협옥잠유상조직분화산생불정아。MS배양기부가저농도6-BA（0.1～0.5mg/L）혹불가6-BA,배합일정농도적생장소（IBA 0.1～0.3 mg/L혹NAA 0.1 mg/L）유리우화협옥잠시관묘장묘생근。생근량호적화협옥잠시관묘이재성활솔체91%±2%。
In this paper,plantlets of Hosta plantaginea were used as explants to investigate the proper conditions for rapid proliferation,growth of plantlets,rooting and transplanting.The results showed that MS medium supplemented with 6-BA(0.1-0.5 mg/L) and IBA(0.01 mg/L) or NAA(0.1 mg/L) were suitable for proliferation of plantlets via in vitro ramet,while MS medium supplemented with 6-BA(≥1.0 mg/L) and IBA(0.01 mg/L) or NAA(0.1 mg/L) were suitable for bud differentiation induced from calli.MS medium supplemented with IBA(0.1-0.3 mg/L) or NAA(0.1 mg/L),with or without low concentrations of 6-BA(0.5 mg/L) were suitable for the growth and rooting of plantlets.Finally,a total survival rate of 91%±2% was obtained for transplanting.
