CAJ | 학술논문

探讨红车轴草异黄酮提取物（red clover extract,RCE）对雄性db/db 2型糖尿病小鼠糖脂代谢的影响。选用6周龄db/db实验小鼠,随机分成三组,每组4只。每日一次定时定量灌胃,每周测其血糖水平。结果表明,用10和50 mg/kg/day剂量的RCE处理小鼠5 w后,血糖水平分别比未处理组显著降低了40.90%（P〈0.01）和50.38%（P〈0.001）,血清甘油三酯比未处理组降低了47.57%（P〈0.01）和43.47%（P〈0.01）,血清总胆固醇比未处理组降低了35.59%（P〈0.05）和35.19%（P〈0.05）。同时实时荧光定量RT-PCR结果显示50 mg/kg/day RCE处理组db/db小鼠肝组织APOC3的mRNA表达水平比未处理组显著下降了54.9%（P〈0.05）。这些结果说明了红车轴草异黄酮提取物可能通过抑制肝APOC3的表达改善2型糖尿病小鼠的血脂水平。
탐토홍차축초이황동제취물（red clover extract,RCE）대웅성db/db 2형당뇨병소서당지대사적영향。선용6주령db/db실험소서,수궤분성삼조,매조4지。매일일차정시정량관위,매주측기혈당수평。결과표명,용10화50 mg/kg/day제량적RCE처리소서5 w후,혈당수평분별비미처리조현저강저료40.90%（P〈0.01）화50.38%（P〈0.001）,혈청감유삼지비미처리조강저료47.57%（P〈0.01）화43.47%（P〈0.01）,혈청총담고순비미처리조강저료35.59%（P〈0.05）화35.19%（P〈0.05）。동시실시형광정량RT-PCR결과현시50 mg/kg/day RCE처리조db/db소서간조직APOC3적mRNA표체수평비미처리조현저하강료54.9%（P〈0.05）。저사결과설명료홍차축초이황동제취물가능통과억제간APOC3적표체개선2형당뇨병소서적혈지수평。
To investigate the effect of red clover extract(RCE) on the blood glucose and lipid metabolism of type 2 diabetic db/db mice,male db/db mice were randomly divided into three groups: db/db mice,db/db + 10 mg /kg/day RCE treated,db/db + 50 mg /kg/day RCE treated.The blood glucose levels of db/db mice receiving 10 mg/kg/day or 50 mg/kg/day RCE for 5 weeks significantly decreased by 40.90%(P0.01) and 50.38%(P0.001) respectively,serum triglycerides decreased by 47.57%(P0.01) and 43.47%(P0.01) respectively,and serum total cholesterols decreased by 35.59%(P0.05) and 35.19%(P0.05) respectively.Moreover,the mRNA expression of hepatic apolipoprotein C3(APOC3) were determined by quantitative real time RT-PCR and 50 mg/kg/day RCE treatment was found to significantly down-regulate APOC3 mRNA expression by 54.9%(P0.05).Our results suggest that RCE significantly improves lipid homeostasis in db/db diabetic mice by down-regulating hepatic APOC3 expression.