贵州科学
貴州科學
귀주과학
GUIZHOU SCIENCE
2012年
5期
23-26
,共4页
麻秀萍%蒋朝晖%贾宪生%张明昶%徐文芬%袁瑶
痳秀萍%蔣朝暉%賈憲生%張明昶%徐文芬%袁瑤
마수평%장조휘%가헌생%장명창%서문분%원요
鞣酸苦参碱%高效液相色谱法%苦参碱%氧化槐果碱%氧化苦参碱
鞣痠苦參堿%高效液相色譜法%苦參堿%氧化槐果堿%氧化苦參堿
유산고삼감%고효액상색보법%고삼감%양화괴과감%양화고삼감
tannin matrine%high performance liquid chromatography%matrine%oxysophocarpine%oxymatrine
为控制鞣酸苦参碱的质量，建立高效液相色谱法（HPLC）测定鞣酸苦参碱中生物碱的含量。方法：采用HPLC法测定鞣酸苦参碱中3个主要生物碱的含量。结果：苦参碱进样量线性范围0．03132～0．1488μg，平均回收率为97．28％，RSD为0．88％；氧化槐果碱线性范围0．01938～0．2132μg，平均回收率为101．1％，RSD为1．03％；氧化苦参碱线性范围0．1751～1．926μg，平均回收率为99．5l％，RSD为0．71％。结论：该法专属性强，准确稳定，重现性好，可作为本品的质量控制方法。
為控製鞣痠苦參堿的質量，建立高效液相色譜法（HPLC）測定鞣痠苦參堿中生物堿的含量。方法：採用HPLC法測定鞣痠苦參堿中3箇主要生物堿的含量。結果：苦參堿進樣量線性範圍0．03132～0．1488μg，平均迴收率為97．28％，RSD為0．88％；氧化槐果堿線性範圍0．01938～0．2132μg，平均迴收率為101．1％，RSD為1．03％；氧化苦參堿線性範圍0．1751～1．926μg，平均迴收率為99．5l％，RSD為0．71％。結論：該法專屬性彊，準確穩定，重現性好，可作為本品的質量控製方法。
위공제유산고삼감적질량，건립고효액상색보법（HPLC）측정유산고삼감중생물감적함량。방법：채용HPLC법측정유산고삼감중3개주요생물감적함량。결과：고삼감진양량선성범위0．03132～0．1488μg，평균회수솔위97．28％，RSD위0．88％；양화괴과감선성범위0．01938～0．2132μg，평균회수솔위101．1％，RSD위1．03％；양화고삼감선성범위0．1751～1．926μg，평균회수솔위99．5l％，RSD위0．71％。결론：해법전속성강，준학은정，중현성호，가작위본품적질량공제방법。
A high performance liquid chromatography (HPLC) was adopted to determine tannin matrine in alka- loid content to control the quality of tannin matrine. Method : HPLC was used for determination of tannin matrine in three main alkaloid contents. Results: The standard curve for matrine was linear in the range of 0. 03132 - 0. 1488 p,g, the average recovery was 97.28 % and RSD was 0.88 %. The standard curve for oxysophocarpine was linear in the range of 0. 01938 - 0. 2132 μg, the average recovery was 101.1% and RSD was 1.03 %. The standard curve for oxymatrine was linear in the range of 0. 1751 - 1. 926 μg, the average recovery was 99.51% and RSD was 0.71%. Conclusion: Being specific, accurate and stable, the method could be used as quality con- trol for tannin matrine.
