CAJ | 학술논문

提出一种基于石蜡电极表面吸附孔雀石绿抗体的电化学免疫传感器，通过竞争型免疫检测法测量水体中残留孔雀石绿。对免疫传感器构制参数——电极表面预处理、抗-MG溶液pH、抗-MG溶液浓度和抗-MG抗体吸附时间进行了研究；对传感器测量过程参数进行了优化，传感器对孔雀石绿检测的线性范围为1．5—780μg／L，检测孔雀石的回收率在93％-116％。
제출일충기우석사전겁표면흡부공작석록항체적전화학면역전감기，통과경쟁형면역검측법측량수체중잔류공작석록。대면역전감기구제삼수——전겁표면예처리、항-MG용액pH、항-MG용액농도화항-MG항체흡부시간진행료연구；대전감기측량과정삼수진행료우화，전감기대공작석록검측적선성범위위1．5—780μg／L，검측공작석적회수솔재93％-116％。
This paper proposes amperometric immunosensor based on the adsorptive immobilization of Malachite Green anti- body on the solid paraffin electrode surface, measuring residues of Malachite Green in water bodies by competitive immuno - detec- ted method. Meanwhile, it studies Fabrication parameters of the immune sensor ( electrode surface pretreatment, pH of anti - MG o- solution, anti - MG solution concentration and anti - MG antibody adsorption time), optimizing parameters of sensor in measuring course, and obtaining 1.5 -780 g/L of Malachite Green measuring range and 93% - 116% of recovery rate.