北京工商大学学报:自然科学版
北京工商大學學報:自然科學版
북경공상대학학보:자연과학판
Journal of Beijing Technology and Business University:Natural Science Edition
2012年
5期
56-61
,共6页
海南毛薯%还原糖%分光光度法
海南毛藷%還原糖%分光光度法
해남모서%환원당%분광광도법
Hainan Dioscorea esculenta (Lour.) brukill%reducing saccharide%spectrophotometry
以葡萄糖为标准溶液,以3,5-二硝基水杨酸为显色剂,用分光光度法测定海南毛薯干粉中总还原糖含量.结果表明:在波长480 nm处有最大吸收峰,测定回收率最大为101.9%,最小为99.7%,相对误差为±1.9%.实测得毛薯干粉中总还原糖含量为71.08%,毛薯中淀粉含量为63.97%.
以葡萄糖為標準溶液,以3,5-二硝基水楊痠為顯色劑,用分光光度法測定海南毛藷榦粉中總還原糖含量.結果錶明:在波長480 nm處有最大吸收峰,測定迴收率最大為101.9%,最小為99.7%,相對誤差為±1.9%.實測得毛藷榦粉中總還原糖含量為71.08%,毛藷中澱粉含量為63.97%.
이포도당위표준용액,이3,5-이초기수양산위현색제,용분광광도법측정해남모서간분중총환원당함량.결과표명:재파장480 nm처유최대흡수봉,측정회수솔최대위101.9%,최소위99.7%,상대오차위±1.9%.실측득모서간분중총환원당함량위71.08%,모서중정분함량위63.97%.
Using glucose as standard and DNS as color developing reagent, the content of total reducing saccharide in Hainan Dioscorea esculenta (Lour.) brukill was determined by spectrophotometry. The re- suits showed that the maximum absorption wavelength of the sample was 480nm. The maximum recovery rate was determined as 101.9% , and the minimum was 99.7%. The relative error was ± 1.9%. The content of total reducing saecharide and starch in Hainan Dioscorea esculenta (Lour.) brukill was 71.08% and 63.97% , respectively.