CAJ | 학술논문

分析了植物组织培养过程中的污染原因,结合外植体消毒技术的研究现状,提出如何控制由外植体引发污染的方法。选择合适的外植体和消毒剂,采用联合消毒方法,对外植体初代培养中内源污染的控制效果比较明显。另外,对于难消毒的外植体,配合使用抗生素能有效地控制污染。
분석료식물조직배양과정중적오염원인,결합외식체소독기술적연구현상,제출여하공제유외식체인발오염적방법。선택합괄적외식체화소독제,채용연합소독방법,대외식체초대배양중내원오염적공제효과비교명현。령외,대우난소독적외식체,배합사용항생소능유효지공제오염。
In this paper, the reason of the pollution in plant tissue culture was analyzed. Combined with the research on the disinfection technology of explants, the method of controlling the pollution caused by the explants was proposed. Selecting the appropriate explants and disinfectants, and using the combined disinfection method, the pollution control of the explants on the initially cultured was effective. In addition, to the explants which were difficultly disinfected, cooperatively using the antibiotics could effectively control the pollution.