畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
ANIMAL HUSBANDRY AND FEED SCIENCE
2012年
9期
21-21,25
,共2页
黄芪精%黄芪甲苷%HPLC-ELSD
黃芪精%黃芪甲苷%HPLC-ELSD
황기정%황기갑감%HPLC-ELSD
Astragalus essence%astragaloside Ⅳ%HPLC-ELSD
采用HPLC-ELSD法对黄芪精中黄芪甲苷的含量进行分析。色谱柱为AgilentC18（5μm,4.6mm×250mm）,柱温为35℃;流动相：乙腈—水（34：66）;流速：1.0mL/min;ELSD检测器参数：漂移管温度为65℃;空气压力为30psi。黄芪甲苷在32.12～321.20μg范围内线性关系良好（r=0.9998）,平均回收率为99.26%,RSD=2.75%（n=6）。该法简便、准确、重现性好,可作为黄芪精质量控制方法。
採用HPLC-ELSD法對黃芪精中黃芪甲苷的含量進行分析。色譜柱為AgilentC18（5μm,4.6mm×250mm）,柱溫為35℃;流動相：乙腈—水（34：66）;流速：1.0mL/min;ELSD檢測器參數：漂移管溫度為65℃;空氣壓力為30psi。黃芪甲苷在32.12～321.20μg範圍內線性關繫良好（r=0.9998）,平均迴收率為99.26%,RSD=2.75%（n=6）。該法簡便、準確、重現性好,可作為黃芪精質量控製方法。
채용HPLC-ELSD법대황기정중황기갑감적함량진행분석。색보주위AgilentC18（5μm,4.6mm×250mm）,주온위35℃;류동상：을정—수（34：66）;류속：1.0mL/min;ELSD검측기삼수：표이관온도위65℃;공기압력위30psi。황기갑감재32.12～321.20μg범위내선성관계량호（r=0.9998）,평균회수솔위99.26%,RSD=2.75%（n=6）。해법간편、준학、중현성호,가작위황기정질량공제방법。
The content of astragaloside Ⅳ in Astragalus essence was determined by HPLC-ELSD.The chromatography column was Agilent C18(5 μm,4.6 mm×250 mm);the column temperature was 35 ℃;the mobile phase was acetonitrile and water(34:66);the flow rate was 1.0 mL/min;the temperature of drift tube of ELSD detector was 65 ℃,and the air pressure was 30 psi.Results showed that the astragaloside Ⅳ within 32.12-321.20 μg had good linear relationship(r=0.999 8);average recovery was 99.26%,and the RSD was 2.75%(n=6).It is suggested that the method is simple and accurate and has good reproducibility,so it could be used in quality control method of Astragalus essence.
