黑龙江农业科学
黑龍江農業科學
흑룡강농업과학
HEILONGJINAG AGRICULTURAL SCIENCE
2014年
2期
45-49
,共5页
巩赫欣%王慧%杨睿%李敏%郭宇萌%商双骄%张荣沭
鞏赫訢%王慧%楊睿%李敏%郭宇萌%商雙驕%張榮沭
공혁흔%왕혜%양예%리민%곽우맹%상쌍교%장영술
棘孢木霉%山新杨%生长量%水解氮含量
棘孢木黴%山新楊%生長量%水解氮含量
극포목매%산신양%생장량%수해담함량
Trichoderma asperellum%Populus davidiana × P .bolleana%growth%available nitrogen content
为了促进木霉免疫诱导剂类生物肥料及土壤改良剂在木本植物生长中的应用,采用盆栽试验方法,以T1(5×102 cfu·cm-3)、T2(5×103 cfu·cm-3)和T3(5×104 cfu·cm-3)3个水平的木霉分生孢子诱导杨树苗60 d ,研究根施棘孢木霉ACCC30536分生孢子悬液后山新杨的生长量及土壤中水解氮含量变化规律。结果表明:T 2水平诱导的效果最佳,杨树苗在15 d内的株高和茎基生长量的最大值分别为7.01 cm和0.15 cm ,是对照CON4(不施木霉)的5.12和2.14倍;单株杨树苗根的平均干重为7.38 g ,较CON4增加50.92%;单株杨树苗茎的平均干重为4.24 g ,较CON4、T1和T3分别增加了16.48%、49.82%和7.34%。同时,以 T2水平木霉诱导杨树苗30 d ,种植杨树苗的盆土中水解氮含量较CON(不施用木霉且不栽培杨树苗的盆土)、CON2(施用T2水平的木霉但不栽培杨树苗的盆土)及CON4分别提高16.81%、9.5%和20.26%。说明棘孢木霉具有极显著促进杨树苗生长和改善土壤中水解氮含量的作用,最佳诱导量为5×103 cf u·cm-3。
為瞭促進木黴免疫誘導劑類生物肥料及土壤改良劑在木本植物生長中的應用,採用盆栽試驗方法,以T1(5×102 cfu·cm-3)、T2(5×103 cfu·cm-3)和T3(5×104 cfu·cm-3)3箇水平的木黴分生孢子誘導楊樹苗60 d ,研究根施棘孢木黴ACCC30536分生孢子懸液後山新楊的生長量及土壤中水解氮含量變化規律。結果錶明:T 2水平誘導的效果最佳,楊樹苗在15 d內的株高和莖基生長量的最大值分彆為7.01 cm和0.15 cm ,是對照CON4(不施木黴)的5.12和2.14倍;單株楊樹苗根的平均榦重為7.38 g ,較CON4增加50.92%;單株楊樹苗莖的平均榦重為4.24 g ,較CON4、T1和T3分彆增加瞭16.48%、49.82%和7.34%。同時,以 T2水平木黴誘導楊樹苗30 d ,種植楊樹苗的盆土中水解氮含量較CON(不施用木黴且不栽培楊樹苗的盆土)、CON2(施用T2水平的木黴但不栽培楊樹苗的盆土)及CON4分彆提高16.81%、9.5%和20.26%。說明棘孢木黴具有極顯著促進楊樹苗生長和改善土壤中水解氮含量的作用,最佳誘導量為5×103 cf u·cm-3。
위료촉진목매면역유도제류생물비료급토양개량제재목본식물생장중적응용,채용분재시험방법,이T1(5×102 cfu·cm-3)、T2(5×103 cfu·cm-3)화T3(5×104 cfu·cm-3)3개수평적목매분생포자유도양수묘60 d ,연구근시극포목매ACCC30536분생포자현액후산신양적생장량급토양중수해담함량변화규률。결과표명:T 2수평유도적효과최가,양수묘재15 d내적주고화경기생장량적최대치분별위7.01 cm화0.15 cm ,시대조CON4(불시목매)적5.12화2.14배;단주양수묘근적평균간중위7.38 g ,교CON4증가50.92%;단주양수묘경적평균간중위4.24 g ,교CON4、T1화T3분별증가료16.48%、49.82%화7.34%。동시,이 T2수평목매유도양수묘30 d ,충식양수묘적분토중수해담함량교CON(불시용목매차불재배양수묘적분토)、CON2(시용T2수평적목매단불재배양수묘적분토)급CON4분별제고16.81%、9.5%화20.26%。설명극포목매구유겁현저촉진양수묘생장화개선토양중수해담함량적작용,최가유도량위5×103 cf u·cm-3。
In order to improve the application of T richoderma asperellum immune-inducer-like bio-fertilizer and soil amendment in benefiting growth of woody plants ,pot culturing experiment was carried out to study the effects of T richoderma asperellum ACCC30536 spore suspension of different concentrations (T1 ,5 × 102 cfu·cm-3 ;T2 ,5×103 cfu·cm-3and T3 ,5×104 cfu·cm-3)on Populus davidiana× P.bolleanagrowth ,the spore suspensions were added to its root to induce the growth for 60 days ,the plants and available nitrogen content in soil were measured .The results showed that T2 induction had significant efficiency ,the seedlings achieved maximum plant height and stem base perimeter increased by 7 .01 cm and 0 .15 cm respectively for 15 days , which were 5 .12 and 2 .14 times more than control CON4(without Trichoderma asperellum treatment) .The average dry root weight of T 2 seedlings was 7 .38 g ,which increased by 50 .92% compared with CON4 ,and av-erage dry stem weight of T2 seedlings was 4 .24 g ,which increased by 16 .48% ,49 .82% and 7 .34% compared with CON4 ,T1 and T3 respectively .Simultaneously ,after inducing for 30 days ,the available nitrogen content of T2 pot soil increased by 16 .81% compared with CON1(pot soil with neither seedling nor inducing ) ,9 .5%compared with CON2(pot soil with inducing as T2 without seedling)and 20 .26% compared with CON4 .The results above showed that T .asperellum had significant effects on the improvement of growth for Shanxinyang and the available nitrogen content of the pot soil ,the best concentration of inducing spore suspention was 5 × 103 cf u · cm -3 .