CAJ | 학술논문

为获得高效转化木糖为乙醇的菌株，以木糖为唯一碳源，通过 TTC 平板显色法从自然环境中进行菌株的初筛，并分别对初筛菌株进行最佳发酵工艺条件的优化；再以相同发酵条件下乙醇产量为复筛指标筛选产醇率最高的菌株，进而对其发酵木糖产乙醇的工艺条件进行正交试验优化。结果表明：初筛共获得4株发酵木糖产乙醇菌株，其中 M3菌株的乙醇得率最高，为22．1％；试验对 M3个体形态以及菌落形态进行了初步鉴定，同时绘制了生长曲线；发酵木糖产乙醇的最佳发酵工艺为木糖浓度30 g/L，发酵时间48 h，初始 pH 5，发酵温度28℃，其乙醇得率达25．3％，比原条件下乙醇得率提高10．0％。
위획득고효전화목당위을순적균주，이목당위유일탄원，통과 TTC 평판현색법종자연배경중진행균주적초사，병분별대초사균주진행최가발효공예조건적우화；재이상동발효조건하을순산량위복사지표사선산순솔최고적균주，진이대기발효목당산을순적공예조건진행정교시험우화。결과표명：초사공획득4주발효목당산을순균주，기중 M3균주적을순득솔최고，위22．1％；시험대 M3개체형태이급균락형태진행료초보감정，동시회제료생장곡선；발효목당산을순적최가발효공예위목당농도30 g/L，발효시간48 h，초시 pH 5，발효온도28℃，기을순득솔체25．3％，비원조건하을순득솔제고10．0％。
In order to obtain strains with high efficient of converting xylose into ethanol,TTC chromogenic screening plate was used to preliminarily screen ethanol-producing strains taking xylose as the single carbon source,and the fermentation process was optimized either.Then the process conditions of fermenting xylose to produce ethanol were optimized through an orthogonal test.The results showed that four strains were gained.Strain M3 got the highest ethanol yield,22.1%.The individual and colony forms of strain M3 were preliminarily identified and the growth curve was drawn;The optimized conditions of fermenting xylose to produce ethanol included xylose concentration 30 g/L,fermentation time 48 h,initial pH 5,fermentation temperature 28℃,ethanol yield 25.3%,10.0% higher than that under original conditions.