CAJ | 학술논문

用研制的连续3批猪伪狂犬病灭活疫苗（HB—J株）分别以不同剂量免疫健康兔和断奶仔猪，检测其免疫28d后的血清抗体，采用血清中和试验法测定血清中和指数，并对免疫28d后的兔和断奶仔猪分别进行攻毒，统计各组兔和断奶仔猪免疫后攻毒保护率。结果表明，兔免疫28d后的血清抗体中和指数为794～1258时保护率为60％～80％，血清抗体中和指数≥1479时可获得100％保护；仔猪免疫28d后的血清抗体中和指数为229～269时保护率为60％～80％，血清抗体中和指数≥331时可获得100％保护。兔与仔猪对猪伪狂犬病灭活疫苗（HB—J株）均产生良好的免疫应答反应，抗体值较高者获得保护率相对较高，兔与猪免疫与攻毒保护呈现平行关系。
용연제적련속3비저위광견병멸활역묘（HB—J주）분별이불동제량면역건강토화단내자저，검측기면역28d후적혈청항체，채용혈청중화시험법측정혈청중화지수，병대면역28d후적토화단내자저분별진행공독，통계각조토화단내자저면역후공독보호솔。결과표명，토면역28d후적혈청항체중화지수위794～1258시보호솔위60％～80％，혈청항체중화지수≥1479시가획득100％보호；자저면역28d후적혈청항체중화지수위229～269시보호솔위60％～80％，혈청항체중화지수≥331시가획득100％보호。토여자저대저위광견병멸활역묘（HB—J주）균산생량호적면역응답반응，항체치교고자획득보호솔상대교고，토여저면역여공독보호정현평행관계。
Healthy rabbits and weanling piglets were immuned respectively with different doses of three successive bathes of swine pseudorabies inactivated vaccine ( HB - J strain). 28 days after inoculation, serum antibodies were detected and healthy rabbits and weanling piglets were challenged respectively by virulent strain of PRV, and the immunity protection rate against virulent virus was tested. The results showed that protection rate in rabbits was from 60% to 80% with the neutralization index of antibodies 794 - 1258, greater than or equal to 1479 with protection rate of 100%; protection rate in piglets was from 60% to 80% with the neutralization index of antibodies 229 -269, greater than or equal to 331 with protection rate 100%. Rabbits and piglets had good immune response to swine pseudorabies inactivated vaccine (HB -J strain). There was good parallel relationship between immunity level and challenge protection.