宁夏农林科技
寧夏農林科技
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NINGXIA JOURNAL OF AGRICULTURE AND FORESTRY SCIENCE AND TECHNOLOGY
2012年
10期
121-123
,共3页
李朔%严如玉%张鹏%易欣欣
李朔%嚴如玉%張鵬%易訢訢
리삭%엄여옥%장붕%역흔흔
微生物肥料%固氮菌%磷细菌%钾细菌%培养
微生物肥料%固氮菌%燐細菌%鉀細菌%培養
미생물비료%고담균%린세균%갑세균%배양
Microbial fertilizer%Fixing-nitrogen bacteria%Phosphorus-dissolving bacteria%Potassium-releasing bacteria%Culture
[目的]筛选出分别可固氮、解磷、解钾的菌株,对其进行鉴定,并利用它们生产微生物肥料。[方法]从供试土壤中分离出目的菌,测定其代谢能力,分别进行16S rDNA测序菌种鉴定,并进行液体培养正交试验,以及尝试固体培养生存复合微生物有机肥。[结果]分离得到代谢能力较强的固氮、解磷、解钾菌N2、P1、K5;鉴定分别为Klebsiella oxytoca、Bacillus cereus和Bacillus mucilaginous;培养条件对有效活菌数的影响大小程度为温度〉C/N〉pH〉转速,最佳培养条件温度32℃,C/N比30,pH 7.0,转速140 r/min,在最佳条件下48 h达到1.78×1010CFU/mL;尝试利用该复合微生物进行固体发酵生产复合微生物有机肥,96h得到有效活菌数2.23×109CFU/g。[结论]筛选出了固氮、解磷及解钾菌株,并对其培养条件进行了优化,为进行生物有机肥生产奠定了基础。
[目的]篩選齣分彆可固氮、解燐、解鉀的菌株,對其進行鑒定,併利用它們生產微生物肥料。[方法]從供試土壤中分離齣目的菌,測定其代謝能力,分彆進行16S rDNA測序菌種鑒定,併進行液體培養正交試驗,以及嘗試固體培養生存複閤微生物有機肥。[結果]分離得到代謝能力較彊的固氮、解燐、解鉀菌N2、P1、K5;鑒定分彆為Klebsiella oxytoca、Bacillus cereus和Bacillus mucilaginous;培養條件對有效活菌數的影響大小程度為溫度〉C/N〉pH〉轉速,最佳培養條件溫度32℃,C/N比30,pH 7.0,轉速140 r/min,在最佳條件下48 h達到1.78×1010CFU/mL;嘗試利用該複閤微生物進行固體髮酵生產複閤微生物有機肥,96h得到有效活菌數2.23×109CFU/g。[結論]篩選齣瞭固氮、解燐及解鉀菌株,併對其培養條件進行瞭優化,為進行生物有機肥生產奠定瞭基礎。
[목적]사선출분별가고담、해린、해갑적균주,대기진행감정,병이용타문생산미생물비료。[방법]종공시토양중분리출목적균,측정기대사능력,분별진행16S rDNA측서균충감정,병진행액체배양정교시험,이급상시고체배양생존복합미생물유궤비。[결과]분리득도대사능력교강적고담、해린、해갑균N2、P1、K5;감정분별위Klebsiella oxytoca、Bacillus cereus화Bacillus mucilaginous;배양조건대유효활균수적영향대소정도위온도〉C/N〉pH〉전속,최가배양조건온도32℃,C/N비30,pH 7.0,전속140 r/min,재최가조건하48 h체도1.78×1010CFU/mL;상시이용해복합미생물진행고체발효생산복합미생물유궤비,96h득도유효활균수2.23×109CFU/g。[결론]사선출료고담、해린급해갑균주,병대기배양조건진행료우화,위진행생물유궤비생산전정료기출。
[Objective]The research aims to screen and identify the strains which have the ability of fixing nitrogen,dissolving phosphorus and releasing potassium,and use these bacteria to produce the microbial fertilizer.[Method]The bacteria were separated from soil,then their related metabolism ability was determined,and their specific 16s rDNA was sequenced.Meanwhile,liquid culture of orthogonal test was carried out and solid culture was used to culture compound microbial fertilizer.[Result] The purpose bacteria N2,P1,K5 with strong metabolism ability were identified to be Klebsiella oxytoca,Bacillus cereus and B.mucilaginous.The effects of culture conditions on effective active bacteria number successively were temperatureC/NpHspeed.The best culture conditions were as follows: temperature 32 ℃,C/N=30,pH=7.0,speed 140 rpm(up to 1.78×1010 CFU/mL after 48 hours under the best condition).The compound microorganism was carried out solid culture to produce compound microbial fertilizer,and the effective active bacteria number was up to 2.23×109 CFU /g after 96 h.[Conclusion] The strains with the ability of fixing nitrogen,dissolving phosphorus and releasing potassium were screened out,and their culture conditions were optimized,which laid the foundation for production of biological organic fertilizer.
