CAJ | 학술논문

建立了测定大鼠肝微粒体温孵体系中血根碱代谢产物二氢血根碱含量的高效液相色谱法。色谱柱为Shimadzu VP-ODSShim-pack（150mm×4．6mm，5μm）；流动相为水：乙腈（37：63，V/V）；流速1mL／min；柱温35℃；检测波长285nm；进样量10μL。结果显示50nmol／L和1000nmol／L二氢血根碱标准溶液的方法回收率分别为101．72％和98．82％；50、100、500nmol／，L标准溶液的日内RSD分别为2．26％、2．57％和3．02％，日间RSD分别为6．21％、4．33％和3．95％：二氢血根碱在25-1000nmol／L内与峰面积呈良好的线性关系（r=0．9996）。本分析方法快速简便，灵敏度高，重现性好，符合方法学验证的要求，可满足二氢血根碱在体外代谢中的定量研究。
건립료측정대서간미립체온부체계중혈근감대사산물이경혈근감함량적고효액상색보법。색보주위Shimadzu VP-ODSShim-pack（150mm×4．6mm，5μm）；류동상위수：을정（37：63，V/V）；류속1mL／min；주온35℃；검측파장285nm；진양량10μL。결과현시50nmol／L화1000nmol／L이경혈근감표준용액적방법회수솔분별위101．72％화98．82％；50、100、500nmol／，L표준용액적일내RSD분별위2．26％、2．57％화3．02％，일간RSD분별위6．21％、4．33％화3．95％：이경혈근감재25-1000nmol／L내여봉면적정량호적선성관계（r=0．9996）。본분석방법쾌속간편，령민도고，중현성호，부합방법학험증적요구，가만족이경혈근감재체외대사중적정량연구。
An HPLC method for the quantitative determination of dihydrosanguinarine in rat liver microsomes incubation system was developed. Chromatography was performed on a Shimadzu VP - ODS column ( 150 mm ×4.6mm,5μm). Acetonitrile -water (63:37, V/V) as the mobile phase was used at 285 nm and 35 ℃. The recovery rate of 50 and 1000 nmol/L standard solution was 101.72% and 98.82% respectively. The relative standard deviation of 50,100 and 500 nmol/L standard solution for the within - day and between - day was 2.26% , 2.57%, 3.02% and 6.21%, 4.33% , 3.95% respectively. The calibration curve was linear within the range from 25 to 1000 nmol/L (r = 0. 9996 ). The results demonstrated that the method was simple, fast and suitable for the quantification of dihydrosanguinarine metabolised in vitro .