河北农业科学
河北農業科學
하북농업과학
JOURNAL OF HEBEI AGRICULTURAL SCIENCES
2012年
2期
62-64
,共3页
王亚艺%李松龄%蔡晓剑%郝玉兰
王亞藝%李鬆齡%蔡曉劍%郝玉蘭
왕아예%리송령%채효검%학옥란
青海%解磷菌%解磷圈%解磷能力%分离%筛选
青海%解燐菌%解燐圈%解燐能力%分離%篩選
청해%해린균%해린권%해린능력%분리%사선
Qinghai%Phosphorus solubilizing bacteria%Dissolved phosphorus ring%Phosphorus solubilizing ability%Isolation%Screening
以青海省主要农耕区海东区和海南区的小麦、洋芋、油菜、蚕豆、辣椒、葱等16种作物的根际土壤为试验材料,通过解磷圈试验对土壤样品进行解磷菌的初筛和复筛。结果表明:共得到具有明显解磷圈的解磷菌28株。通过对解磷圈大小测定,筛选出y8-4、y5.1和w4.1等9株菌表现较好,解磷圈直径(D)与菌落直径(d)比值(D/d)均≥1.5。通过摇瓶复筛得出,y8-4解磷能力较强,菌液中磷含量达95μg/mL,其次是菌株y16-4和y9-4;其中菌株y9-4培养液的pH值有所降低。综合评价认为菌株y9-4的彤d较大,培养液中磷含量较高,且pH值有所降低,最后确定其作为适合青海农作区推广的高效解磷菌。
以青海省主要農耕區海東區和海南區的小麥、洋芋、油菜、蠶豆、辣椒、蔥等16種作物的根際土壤為試驗材料,通過解燐圈試驗對土壤樣品進行解燐菌的初篩和複篩。結果錶明:共得到具有明顯解燐圈的解燐菌28株。通過對解燐圈大小測定,篩選齣y8-4、y5.1和w4.1等9株菌錶現較好,解燐圈直徑(D)與菌落直徑(d)比值(D/d)均≥1.5。通過搖瓶複篩得齣,y8-4解燐能力較彊,菌液中燐含量達95μg/mL,其次是菌株y16-4和y9-4;其中菌株y9-4培養液的pH值有所降低。綜閤評價認為菌株y9-4的彤d較大,培養液中燐含量較高,且pH值有所降低,最後確定其作為適閤青海農作區推廣的高效解燐菌。
이청해성주요농경구해동구화해남구적소맥、양우、유채、잠두、랄초、총등16충작물적근제토양위시험재료,통과해린권시험대토양양품진행해린균적초사화복사。결과표명:공득도구유명현해린권적해린균28주。통과대해린권대소측정,사선출y8-4、y5.1화w4.1등9주균표현교호,해린권직경(D)여균락직경(d)비치(D/d)균≥1.5。통과요병복사득출,y8-4해린능력교강,균액중린함량체95μg/mL,기차시균주y16-4화y9-4;기중균주y9-4배양액적pH치유소강저。종합평개인위균주y9-4적동d교대,배양액중린함량교고,차pH치유소강저,최후학정기작위괄합청해농작구추엄적고효해린균。
The phosphorus-solubilizing bacteria were isolated and screened from the rhizosphere soil of 16 crops in Qinghai Province. The results showed that 28 strains were obtained which had obviously dissolved phosphorus ring. By determining the ring size, 9 strains were screened with the ratio of dissolving phosphate zone diameter to bacterial colony diameter (D/d) greater than or equal to 1.5. The phosphate-dissolving ability of strain y84 was higher, than y64 and y94. pH value of y9-4 culture solution decreased. Based on the comprehensive evaluation, strain y9-4 with higher D/d value, higher phosphorus content in the culture medium and decreased pH, which was suitable for popularizing in agricuhural district in Qinghai