甘蔗SPSⅢ基因启动子5’侧翼缺失的GUS表达载体构建
감자SPSⅢ기인계동자5’측익결실적GUS표체재체구건
The Construction of Sugarcane SPSⅢ Promoter 5＇ Flank Sequence Deletion Vectors for GUS Gene Expression
  • 万方数据
    福建教育学院学报 복건교육학원학보
    JOURNAL OF FUJIAN EDUCATION INSTITUTE
    2012年 3期 118-121 ,共4页

    张积森%郑月霞%方静平%高玉娜%郭春芳%叶冰莹%陈由强%陈如凯

    장적삼%정월하%방정평%고옥나%곽춘방%협빙형%진유강%진여개

    甘蔗%蔗糖磷酸合成酶基因Ⅲ%5′端缺失表达载体%GUS基因 甘蔗%蔗糖燐痠閤成酶基因Ⅲ%5′耑缺失錶達載體%GUS基因
    감자%자당린산합성매기인Ⅲ%5′단결실표체재체%GUS기인
    Saccharum Spp.%Sucrose Phosphate Synthase%5'Flank Sequence Deletion Vectors%GUS gene
    蔗糖磷酸合成酶是调节植物蔗糖合成的关键酶之一。本研究根据该段序列上预测的顺式作用元件将SPSⅢ5＇侧翼序列不同长度的片段与GUS基因融合,成功构建了3个缺失表达载体,为进一步的甘蔗SPSⅢ启动子活性区域鉴定提供基础。
    자당린산합성매시조절식물자당합성적관건매지일。본연구근거해단서렬상예측적순식작용원건장SPSⅢ5＇측익서렬불동장도적편단여GUS기인융합,성공구건료3개결실표체재체,위진일보적감자SPSⅢ계동자활성구역감정제공기출。
    Sucrose phosphate synthase is considered to be the key enzymes in the sucrose metabolism. In the study, for further investigating the functional region the SPS Ⅲ promoter in sugarcane, 3 flank sequence deletion vectors for GUS gene expression were constructed using pCAMBIA 1391z.
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