CAJ | 학술논문

以单分散交联聚甲基丙烯酸环氧丙酯（PGMA/EDMA）微球作为基质载体,溶菌酶为模板分子,β-环糊精和丙烯酰胺为复合单体,N,N′-亚甲基双丙烯酰胺为交联剂,采用表面印迹法制备了溶菌酶分子印迹聚合物;以此聚合物为固定相进行前沿色谱分析和识别性能研究.结果表明,该分子印迹聚合物对模板分子溶菌酶具有特异识别能力,基于此可以分别实现溶菌酶与牛血清白蛋白、卵白蛋白及肌红蛋白的色谱分离.
이단분산교련취갑기병희산배양병지（PGMA/EDMA）미구작위기질재체,용균매위모판분자,β-배호정화병희선알위복합단체,N,N′-아갑기쌍병희선알위교련제,채용표면인적법제비료용균매분자인적취합물;이차취합물위고정상진행전연색보분석화식별성능연구.결과표명,해분자인적취합물대모판분자용균매구유특이식별능력,기우차가이분별실현용균매여우혈청백단백、란백단백급기홍단백적색보분리.
Lysozyme-based molecularly imprinted polymers （Lys-MIPs） were prepared with POMA/EDMA beads as a carrier material, lysozyme as the template molecule, β-cyclodextrin and acrylamide as the double functional monomers, N,N＇-methylene bisacrylamide as the crosslinker. The prepared Lys-MIPs were used as a new HPLC packing material, based on which the related study on its recognition was conducted through the frontier chromatography. The results showed that Lys-MIPs had specific recognition for lysozyme, which helped achieve the separation between lysozyme and three individual competitive proteins, including bovine serum albumin （BSA）, ovalbumin（OB） and myoglobin （Myo）.