CAJ | 학술논문

从常温研磨、蛋白酶K和R nase的加入对传统CTAB法进行改良,并结合试剂盒法,提出了一种高效提取高温大曲微生物总DNA的方法。该方法获得的总基因片段大小约21Kb;A260/A280=1.878;A260/A230=1.706;PCR反应抑制物少,可直接用于16S rDNA的扩增;并通过构建克隆文库技术和变性梯度凝胶电泳（DGGE）分析后发现高温大曲中细菌多样性丰富,能一定程度上反映微生物群落结构和组成。研究结果表明该方法提取的DNA适用于芝麻香高温大曲中微生物的分子生态学研究。
종상온연마、단백매K화R nase적가입대전통CTAB법진행개량,병결합시제합법,제출료일충고효제취고온대곡미생물총DNA적방법。해방법획득적총기인편단대소약21Kb;A260/A280=1.878;A260/A230=1.706;PCR반응억제물소,가직접용우16S rDNA적확증;병통과구건극륭문고기술화변성제도응효전영（DGGE）분석후발현고온대곡중세균다양성봉부,능일정정도상반영미생물군락결구화조성。연구결과표명해방법제취적DNA괄용우지마향고온대곡중미생물적분자생태학연구。
This paper conducted a research on the methods of DNA extraction and defined an mneliorative method. The obtained total genome fragment extracted with this method was about 21kb in size and the purity was A260/A280=1.878, A260/A230=1.706, and can be directly applied to PCR amplification of 16S rDNA. With further validation by cloning library and DGGE, the DNA can represent the microbe community. These resuhs indicate that the total DNA extracted with this method applies to the study on the molecular biology of high temperature Daqu.