CAJ | 학술논문

观察KGF-1二硫键突变体对人肝星状细胞的抑制作用和其对试验性肝损伤的修复作用,同时探讨二硫键对蛋白质生物活性的影响。运用CCl4诱导的肝纤维化模型对△23KGF-1组、△23Ser102 KGF-1组（不含二硫键）和△23Ser102 C40-C106 KGF-1组（含二硫键）进行体内抗纤维化活性的比较,并研究其对人肝星状细胞系的抑制作用。结果表明,与模型组相比,KGF-1突变体的各项指标均优于模型组,并且△23Ser102 KGF-1可明显抑制人肝星状细胞系的生长,可见KGF-1突变体不含二硫键组对大鼠试验性肝损伤有明显保护作用。此外实验还证明二硫键的有无会直接影响蛋白质的生物活性。
관찰KGF-1이류건돌변체대인간성상세포적억제작용화기대시험성간손상적수복작용,동시탐토이류건대단백질생물활성적영향。운용CCl4유도적간섬유화모형대△23KGF-1조、△23Ser102 KGF-1조（불함이류건）화△23Ser102 C40-C106 KGF-1조（함이류건）진행체내항섬유화활성적비교,병연구기대인간성상세포계적억제작용。결과표명,여모형조상비,KGF-1돌변체적각항지표균우우모형조,병차△23Ser102 KGF-1가명현억제인간성상세포계적생장,가견KGF-1돌변체불함이류건조대대서시험성간손상유명현보호작용。차외실험환증명이류건적유무회직접영향단백질적생물활성。
The study observed the inhibition and anti-fibrosis effect of KGF-1 mutant protein on rats hepatic fibrosis induced by CCl4 model, and discussed the relationship of its disulfide bond and func- tion. The SD rats CCl4 model with liver fibrosis were utilized to evaluate the bioactivities of KGF-1 deleted 23 amino acids, KGF-1 mutant with no disulfide bond and KGF-1 mutant with Cys40-Cysl02 disulfide bond. HSC would be used to evaluate the bioactivities of them. Compared with the model group, the biochemical indicator of controlled group was much better. KGF-1 mutant with no disulfide bond has the obvious effect on experimental liver damage, which proved that disulfide bond could affect the bioactivity of protein directly.