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农业科学与技术：英文版 농업과학여기술：영문판
Agricultural Science ＆ Technology
2012年 5期 1021-1027 ,共7页

王玲%李昆%孟银萍%赵丽雅%李兆华

왕령%리곤%맹은평%조려아%리조화

根际微生物%丛枝菌根真菌%分子多样性%有机基质%保护地栽培%甜椒%变性梯度凝胶电泳%土壤添加剂根際微生物%叢枝菌根真菌%分子多樣性%有機基質%保護地栽培%甜椒%變性梯度凝膠電泳%土壤添加劑
근제미생물%총지균근진균%분자다양성%유궤기질%보호지재배%첨초%변성제도응효전영%토양첨가제
Arbuscular mycorrhizal fungi%Organic soil substrate%Denaturing gradient gel electrophoresis%Microbial diversity%Sweet pepper

Denaturing gradient gel electrophoresis （DGGE） was used to study the ef- fects of different soil additives, Le. control soil ＋ Glomus mossea-2 （G.M）, organic substrate, organic substrate ＋ G.M, on microbial molecular diversity in sweet pepper rhizosphere under protected cultivation. Results showed that following the inoculation of G.M, the total number of bacterial species increased, with the appearance and disappearance of some bacterial species. Some bacteria, though detected in all treatments, showed increased cell/clone numbers with the addition of arbuscular myc- orrhizal fungus G.M. The organic substrate favored the growth and propagation of some bacteria and was advantageous to the establishment of some dominant micro- bial flora. Resulted from the similarity analyses of the DGGE profile, it was obvious that organic substrate had more influence on the diversity of rhizosphere microbial flora than the inoculation of arbuscular mycorrhizal fungus G.M. Meanwhile, bacterial diversity in the control soil inoculated with G.M was higher than the organic soil. It was thus concluded that the inoculation of G.M changed the structure of microbial population in sweet pepper rhizosphere and increased the similarity of the rhizo- sphere bacterial species.