CAJ | 학술논문

以芦丁为标准品，建立了紫外分光光度法测定苦槛蓝黄酮含量的方法，通过单因素与正交实验研究了苦槛蓝叶中黄酮的提取工艺。结果表明，采用AlCl3显色法适合苦槛蓝黄酮含量的测定，其稳定性、精密度、回收率的相对标准偏差RSD（relativestandarddeviation）分别为0．506％、130％、4．20％。影响苦槛蓝黄酮得率的主次因素为：温度〉乙醇体积分数〉提取时间〉料液比，最佳提取工艺条件为：25倍最的60％的乙醇，在温度为90℃的条件下，提取2．0h，提取3次，苦槛蓝总黄酮平均收率为5．16％。
이호정위표준품，건립료자외분광광도법측정고함람황동함량적방법，통과단인소여정교실험연구료고함람협중황동적제취공예。결과표명，채용AlCl3현색법괄합고함람황동함량적측정，기은정성、정밀도、회수솔적상대표준편차RSD（relativestandarddeviation）분별위0．506％、130％、4．20％。영향고함람황동득솔적주차인소위：온도〉을순체적분수〉제취시간〉료액비，최가제취공예조건위：25배최적60％적을순，재온도위90℃적조건하，제취2．0h，제취3차，고함람총황동평균수솔위5．16％。
The content of flavonoids ofmyoporum bontioides A. Gray was determined by using ultraviolet spectrophotometry, rutin as the standard substance, and the extraction conditions of flavonoids ofmyoporum bontioides A. Gray were studied by using orthogonal test. The results showed that the AICI3 was suitable for the determination of the flavonoids content and the RSD of stability and precision was 0.506 %, 1.30 %, 4.20 %, respectively. And the factors affecting the extraction quality were in the order of the extracting temperature〉ethanol concentration〉extracting time〉ratio of solid to liquid, and the yield of flavonoids of myoporum bontioides A. Gray was up to 5.15 % at the optimal extraction conditions with 60 % of ethanol and solid to liquid ratio of 1 ： 25 at the 90 ℃, which were extracted three times, 2 h per time.