广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2012年
8期
133-134,142
,共3页
何艳%陈华国%赵超%周欣%王垄%申海艳
何豔%陳華國%趙超%週訢%王壟%申海豔
하염%진화국%조초%주흔%왕롱%신해염
黄芪颗粒%总黄酮%紫外分光光度法
黃芪顆粒%總黃酮%紫外分光光度法
황기과립%총황동%자외분광광도법
Huangqi Granule%total flavone%UV spectrophotometry
建立了黄芪颗粒中总黄酮的含量测定方法:以芦丁为参照,利用紫外分光光度法在371 nm波长测定黄芪颗粒中总黄酮的含量。芦丁在5.90~59.0μg/mL线性关系良好,线性方程为A=0.0103C+0.2707,γ=0.9963,平均回收率为98.65%,RSD%=0.85%。结果表明:紫外分光光度法测定黄芪颗粒中总黄酮的含量方法简便,快捷,准确,可用来做黄芪颗粒的质量评估的方法。
建立瞭黃芪顆粒中總黃酮的含量測定方法:以蘆丁為參照,利用紫外分光光度法在371 nm波長測定黃芪顆粒中總黃酮的含量。蘆丁在5.90~59.0μg/mL線性關繫良好,線性方程為A=0.0103C+0.2707,γ=0.9963,平均迴收率為98.65%,RSD%=0.85%。結果錶明:紫外分光光度法測定黃芪顆粒中總黃酮的含量方法簡便,快捷,準確,可用來做黃芪顆粒的質量評估的方法。
건립료황기과립중총황동적함량측정방법:이호정위삼조,이용자외분광광도법재371 nm파장측정황기과립중총황동적함량。호정재5.90~59.0μg/mL선성관계량호,선성방정위A=0.0103C+0.2707,γ=0.9963,평균회수솔위98.65%,RSD%=0.85%。결과표명:자외분광광도법측정황기과립중총황동적함량방법간편,쾌첩,준학,가용래주황기과립적질량평고적방법。
A new method for determination the total flavonoids in Huangqi Granule was established. The total fla- vonoids in Huangqi Granule were determined by UV spectrophotometry using rutin as the reference at the wavelength of 371 nm. The linear equation was A = 0.0103C + 0.2707, γ/= 0.9963, and linear range of calibration curve was 5.9 59 μg/mL. The average recovery rate was 98.65% with the relative standard deviation 0.86%. The method of UV spec- trophotometry determination the total flavonoids in Huangqi Granule was simple, fast and accurate, which can be used to evaluate the quality of Huangqi Granule.