CAJ | 학술논문

为了探索香泽兰总黄酮对斑马鱼脾脏显微组织结构的毒性作用，采集香泽兰全草，用70％乙醇（v／v）浸泡后，辅以超声提取，再经大孔树脂柱层析纯化，浓缩，冰冻干燥，制得香泽兰总黄酮冻干粉，用此总黄酮冻干粉按不同浓度对瘫马鱼进行水触媒染毒，21d后取脾脏，制作石蜡切片，苏木精一伊红染色，显微观察。结果发现，随着香泽兰总黄酮水触媒染毒浓度的增加，供试斑马鱼脾脏显微组织结构出现了不同程度的病变，主要表现含铁血黄素沉着增多，脾窦扩张，脾脏内淋巴细胞增多，血细胞核浓缩、溶解，甚至导致脾脏坏死。说明一定浓度的香泽兰总黄酮对宽马鱼脾脏具有一定的毒性作用。
위료탐색향택란총황동대반마어비장현미조직결구적독성작용，채집향택란전초，용70％을순（v／v）침포후，보이초성제취，재경대공수지주층석순화，농축，빙동간조，제득향택란총황동동간분，용차총황동동간분안불동농도대탄마어진행수촉매염독，21d후취비장，제작석사절편，소목정일이홍염색，현미관찰。결과발현，수착향택란총황동수촉매염독농도적증가，공시반마어비장현미조직결구출현료불동정도적병변，주요표현함철혈황소침착증다，비두확장，비장내림파세포증다，혈세포핵농축、용해，심지도치비장배사。설명일정농도적향택란총황동대관마어비장구유일정적독성작용。
To probe into the micro-structural changes of spleen of zebrafish exposure to flavonoids extracted from Eupatorium odoratum Linn.. the whole natural E.odoratum was collected, dried and ground, then its flavonoids were extracted by 70% ethanol （v/v） marinating and ultrasonic technology. Afterwards, the extract was purified by macroporous resin and made into frozen-dried powder. Different concentrations cff the frozen-dried powder of flavonoids were exposed to zebrafish （Danio rerio） by water pollution. After 21 days of exposure, the spleen of the zebrafish was collected to make paraffin-clothed and hematnxylin-eosin stained slides, and the histopathological changes were observed. It was found that, with the dosage increasing of the flavonoids, the microstructure of spleen of the zebrafish exposed to the flavonoids appeared pathological changes as follows： increase of hemosiderosis, dilated splenic sinus, increase of lymphocytes, condensed and dissolved nuclear of blood cell, and even necrosis of spleen. It was concluded that certain concentrations of flavonoids extracted from E.odoratum caused certain toxicities to spleen of zehrafish.