现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2012年
17期
277-278
,共2页
苏丹红Ⅰ%苏丹红Ⅱ%高效液相法%辣椒精油%测定
囌丹紅Ⅰ%囌丹紅Ⅱ%高效液相法%辣椒精油%測定
소단홍Ⅰ%소단홍Ⅱ%고효액상법%랄초정유%측정
Sudan Red Ⅰ%Sudan Red Ⅱ%HPLC%pepper essential oil%determination
采用1%冰醋酸水溶液、1%冰醋酸溶液、20%丙酮乙腈溶液分别进行梯度洗脱,测定苏丹红Ⅰ和苏丹红Ⅱ的含量。结果表明,苏丹红Ⅰ测定的线性范围为0～1.6μg/mL,线性相关系数为0.998 9,苏丹红Ⅱ测定的线性范围为0～1.6μg/mL,线性相关系数为0.994 7。苏丹红Ⅰ的加标回收率为89.38%,苏丹红Ⅱ的加标回收率为90.27%。该方法灵敏度高,结果准确且稳定。
採用1%冰醋痠水溶液、1%冰醋痠溶液、20%丙酮乙腈溶液分彆進行梯度洗脫,測定囌丹紅Ⅰ和囌丹紅Ⅱ的含量。結果錶明,囌丹紅Ⅰ測定的線性範圍為0～1.6μg/mL,線性相關繫數為0.998 9,囌丹紅Ⅱ測定的線性範圍為0～1.6μg/mL,線性相關繫數為0.994 7。囌丹紅Ⅰ的加標迴收率為89.38%,囌丹紅Ⅱ的加標迴收率為90.27%。該方法靈敏度高,結果準確且穩定。
채용1%빙작산수용액、1%빙작산용액、20%병동을정용액분별진행제도세탈,측정소단홍Ⅰ화소단홍Ⅱ적함량。결과표명,소단홍Ⅰ측정적선성범위위0～1.6μg/mL,선성상관계수위0.998 9,소단홍Ⅱ측정적선성범위위0～1.6μg/mL,선성상관계수위0.994 7。소단홍Ⅰ적가표회수솔위89.38%,소단홍Ⅱ적가표회수솔위90.27%。해방법령민도고,결과준학차은정。
1% glacial acetic acid aqueous,1% glacial acetic acid and 20% acetone acetonitrile solution were used as mobile phase for the gradient elution to determinate the content of Sudan Red Ⅰ and Sudan Red Ⅱ by HPLC.The results showed that the linear range of Sudan Red Ⅰ was 0～1.6 μg/mL,and the linearly dependent coefficient of Sudan Red Ⅰwas 0.998 9.The linear range of Sudan Red Ⅱ was 0～1.6 μg/mL,and the linearly dependent coefficient of Sudan Red Ⅱ was 0.994 7.The recovery rate of Sudan Red Ⅰ was 89.38%,and the recovery rate of Sudan Red Ⅱwas 90.27%.The method was high sensitivity and the results was accurate and stable.
