中国调味品
中國調味品
중국조미품
CHINESE CONDIMENT
2014年
4期
112-115
,共4页
褪色光度法%钴%酵母
褪色光度法%鈷%酵母
퇴색광도법%고%효모
fading spectrophotometry%cobalt%yeast
研究钴（Ⅱ）与硫氰酸钾和亮绿的络合反应，建立褪色光度法测定钴含量的新方法。通过试验优化酸度、硫氰酸钾用量、亮绿用量、反应温度和反应时间等测定条件。结果表明：最大褪色波长为624 nm，表观摩尔吸光系数为2．2×105 L/mol·cm，钴（Ⅱ）浓度在0～0．30μg/mL范围内服从比尔定律，检出限为2．5μg/L。方法用于测定酵母中钴含量，结果与原子吸收光谱法一致，相对标准偏差为0．53％～0．81％（n ＝5），回收率为98．1％～101．5％。
研究鈷（Ⅱ）與硫氰痠鉀和亮綠的絡閤反應，建立褪色光度法測定鈷含量的新方法。通過試驗優化痠度、硫氰痠鉀用量、亮綠用量、反應溫度和反應時間等測定條件。結果錶明：最大褪色波長為624 nm，錶觀摩爾吸光繫數為2．2×105 L/mol·cm，鈷（Ⅱ）濃度在0～0．30μg/mL範圍內服從比爾定律，檢齣限為2．5μg/L。方法用于測定酵母中鈷含量，結果與原子吸收光譜法一緻，相對標準偏差為0．53％～0．81％（n ＝5），迴收率為98．1％～101．5％。
연구고（Ⅱ）여류청산갑화량록적락합반응，건립퇴색광도법측정고함량적신방법。통과시험우화산도、류청산갑용량、량록용량、반응온도화반응시간등측정조건。결과표명：최대퇴색파장위624 nm，표관마이흡광계수위2．2×105 L/mol·cm，고（Ⅱ）농도재0～0．30μg/mL범위내복종비이정률，검출한위2．5μg/L。방법용우측정효모중고함량，결과여원자흡수광보법일치，상대표준편차위0．53％～0．81％（n ＝5），회수솔위98．1％～101．5％。
Study the complexation reaction of Co (Ⅱ)-potassium thiocyanate-light green system,a new fading spectrophotometry for determination of cobalt content is established.The optimal determina-tion conditions are studied after optimization of acidity,dosage of potassium thiocyanate and bright green,reaction temperature and time by experiments.The results show that the maximum fading wavelength is at 624 nm,the apparent molar absorption coefficient is 2.2×105 L/mol·cm,the cobalt (II)content within the range of 0~0.30μg/mL obeys Beer's law,the detection limit is 2.5μg/L. This method is applied for the determination of cobalt content in yeast.The results obtained by this method are consistent with those obtained by atomic absorption spectrometry.The RSD is in the range of 0.53%~0.81% (n= 5),and the recovery is in the range of 98.1%~101.5%.
