CAJ | 학술논문

目的构建针对大鼠结缔组织生长因子（connective tissue growth factor ，CTGF）的小分子干扰RNA（smal interfering RNA，siRNA）荧光逆转录病毒表达载体。方法将体外合成的两对各段含65碱基的寡核苷酸连接到pSIREN-RetroQ-ZsGreen载体，构建重组质粒，同时设立非特异对照。结果酶切、DNA序列鉴定均证实插入片段的正确性。结论成功地构建了鼠CTGF-siRNA荧光逆转录病毒表达载体。为进一步研究其对体内外抗纤维化作用打下基础。
목적구건침대대서결체조직생장인자（connective tissue growth factor ，CTGF）적소분자간우RNA（smal interfering RNA，siRNA）형광역전록병독표체재체。방법장체외합성적량대각단함65감기적과핵감산련접도pSIREN-RetroQ-ZsGreen재체，구건중조질립，동시설립비특이대조。결과매절、DNA서렬감정균증실삽입편단적정학성。결론성공지구건료서CTGF-siRNA형광역전록병독표체재체。위진일보연구기대체내외항섬유화작용타하기출。
Objective To design and construct retrovirus expressing smal interference RNAs (siRNAs) targeting rat us connective tissue growth factor(CTGF)mediated by pSIREN-RetroQ-ZsGreen vector. Methods Four pairs of 65 base-pair oligonucleotides were synthesized in vivo and inserted in pSIREN-RetroQ-ZsGreen vector, promoter to construct recombinant plasmids. Oligos with a scrambled sequence w ere used as a negat ive control. Results Correct construction of CTGF siRNAs vectors was identified by methods of restriction enzyme analysis , and nucleotide sequence determination. Conclusion The fluorescent retrovirus expression vectors of CTGF siRNAs were constructed successful y.This wil facilitate the stuy of antifibrotic intervention in vivo and in vitro.