CAJ | 학술논문

目的：考察 G2.0聚酰胺-胺树状大分子(PAMAM)及水杨醛修饰物(PAMAM-SA)的释药机制。方法用紫外可见分光光度法检测2种大分子环境敏感性及对氧氟沙星的增溶作用。氧氟沙星含量测定采用 HPLC 法：ODS-C18色谱柱(150 mm×4.6 mm,5μm)；流动相为乙腈、三乙胺溶液(磷酸调 pH 4.0)(14∶86)；检测波长294 nm；流速1.0 mL·min-1；柱温40℃。结果氧氟沙星单独释放80%需要2 h,G2.0 PAMAM-氧氟沙星复合物释放氧氟沙星80%需要5 h,G2.0 PAMAM-SA-氧氟沙星复合物释放氧氟沙星80%需要2.5 h。结论2种大分子具有环境敏感性,对氧氟沙星具有增溶、缓释作用,2种复合物形成是大分子的氨基阳离子与氧氟沙星的羧基阴离子之间静电作用的结果。
목적：고찰 G2.0취선알-알수상대분자(PAMAM)급수양철수식물(PAMAM-SA)적석약궤제。방법용자외가견분광광도법검측2충대분자배경민감성급대양불사성적증용작용。양불사성함량측정채용 HPLC 법：ODS-C18색보주(150 mm×4.6 mm,5μm)；류동상위을정、삼을알용액(린산조 pH 4.0)(14∶86)；검측파장294 nm；류속1.0 mL·min-1；주온40℃。결과양불사성단독석방80%수요2 h,G2.0 PAMAM-양불사성복합물석방양불사성80%수요5 h,G2.0 PAMAM-SA-양불사성복합물석방양불사성80%수요2.5 h。결론2충대분자구유배경민감성,대양불사성구유증용、완석작용,2충복합물형성시대분자적안기양리자여양불사성적최기음리자지간정전작용적결과。
Objective To investigate the release mechanism of G2.0 PAMAM and G2.0 PAMAM-SA.Methods The UV spectro-photometry was employed to measure the environmental sensitivity of G2.0 PAMAM and G2.0 PAMAM-SA and their effects on the solubility enhancement of ofloxacin (OFL).The content of OFL was measured by an HPLC method with an ODS-C18 chroma-tographic column(150 mm × 4.6 mm,5μm)which was kept at 40 ℃,with a mobile phase of acetonitrile-triethylamine (14∶86, adjusting pH to 4.0 by phosphoric acid)at a flow rate of 1.0 mL ·min-1 and the detection wavelength was 294 nm.Results It took 2 h for 80% OFL to release alone,5 h to release 80% OFL from G2.0 PAMAM-OFL compound and 2.5 h to release 80%OFL from G2.0 PAMAM-SA-OFL compound.Conclusion G2.0 PAMAM and G2.0 PAMAM-SA are environmentally sensitive, and it is an effective way to enhance the solubility and suspend the release of OFL.The formations of the two compounds may re-sult from the interaction between the amino group of PAMAMs and the carboxyl group of OFL.