西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
1期
31-34
,共4页
链麻滴鼻液%HPLC%硫酸链霉素%盐酸麻黄碱
鏈痳滴鼻液%HPLC%硫痠鏈黴素%鹽痠痳黃堿
련마적비액%HPLC%류산련매소%염산마황감
Streptomycin Sulfate and Ephedrine Hydrochloride Nasal Drops%HPLC%streptomycin sulfate%ephedrine hydrochloride
目的：建立测定链麻滴鼻液中硫酸链霉素和盐酸麻黄碱含量的高效液相色谱法,消除焦亚硫酸钠对硫酸链霉素含量测定的影响。方法色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm)；流动相为0.014 mol·L-1庚烷磺酸钠的0.05 mol·L-1磷酸二氢钾溶液-乙腈(78∶22)；检测波长硫酸链霉素195 nm,盐酸麻黄碱207 nm；流速1.0 mL·min-1。结果硫酸链霉素质量浓度在44.40~310.80μg·mL-1范围内,与其峰面积呈良好的线性关系。(r=0.9998,n=7),平均回收率为103.4%(RSD=3.4%)；盐酸麻黄碱质量浓度在20.224~80.896μg·mL-1范围内,与其峰面积呈良好的线性关系(r=0.9999,n=7),平均回收率为99.5%(RSD=1.0%)。结论该方法专属性强、测定结果准确、精密度高、线性好,加入甲醛能够消除辅料中焦亚硫酸钠对硫酸链霉素含量测定的影响。
目的：建立測定鏈痳滴鼻液中硫痠鏈黴素和鹽痠痳黃堿含量的高效液相色譜法,消除焦亞硫痠鈉對硫痠鏈黴素含量測定的影響。方法色譜柱為Diamonsil C18柱(250 mm×4.6 mm,5μm)；流動相為0.014 mol·L-1庚烷磺痠鈉的0.05 mol·L-1燐痠二氫鉀溶液-乙腈(78∶22)；檢測波長硫痠鏈黴素195 nm,鹽痠痳黃堿207 nm；流速1.0 mL·min-1。結果硫痠鏈黴素質量濃度在44.40~310.80μg·mL-1範圍內,與其峰麵積呈良好的線性關繫。(r=0.9998,n=7),平均迴收率為103.4%(RSD=3.4%)；鹽痠痳黃堿質量濃度在20.224~80.896μg·mL-1範圍內,與其峰麵積呈良好的線性關繫(r=0.9999,n=7),平均迴收率為99.5%(RSD=1.0%)。結論該方法專屬性彊、測定結果準確、精密度高、線性好,加入甲醛能夠消除輔料中焦亞硫痠鈉對硫痠鏈黴素含量測定的影響。
목적：건립측정련마적비액중류산련매소화염산마황감함량적고효액상색보법,소제초아류산납대류산련매소함량측정적영향。방법색보주위Diamonsil C18주(250 mm×4.6 mm,5μm)；류동상위0.014 mol·L-1경완광산납적0.05 mol·L-1린산이경갑용액-을정(78∶22)；검측파장류산련매소195 nm,염산마황감207 nm；류속1.0 mL·min-1。결과류산련매소질량농도재44.40~310.80μg·mL-1범위내,여기봉면적정량호적선성관계。(r=0.9998,n=7),평균회수솔위103.4%(RSD=3.4%)；염산마황감질량농도재20.224~80.896μg·mL-1범위내,여기봉면적정량호적선성관계(r=0.9999,n=7),평균회수솔위99.5%(RSD=1.0%)。결론해방법전속성강、측정결과준학、정밀도고、선성호,가입갑철능구소제보료중초아류산납대류산련매소함량측정적영향。
Obj ective To determine the contents of streptomycin sulfate and ephedrine hydrochloride in Streptomycin Sulfate and E-phedrine Hydrochloride Nasal Drops by HPLC,and to eliminate the affect of sodium metabisulfite on the content determination of streptomycin sulfate.Method The stationary phase was Diamonsil C18 (250 mm×4.6 mm,5 μm);the mobile phase was 0.05 mol·L-1 potassium dihydrogen phosphate solution containing 0.014 mol·L-1 sodium heptanesulfonate and acetonitrile (78∶22);the detection wavelength of streptomycin sulfate was 195 nm,and ephedrine hydrochloride was 207 nm;the flow rate was 1.0 mL·min-1 .Results The linear range for streptomycin sulfate was 44.40-310.80μg·mL-1 (r=0.999 8,n=7),and the av-erage recovery was 103.4% (RSD=3.4% );The linear range for ephedrine hydrochloride was 20.224-80.896 μg·mL-1 (r=0.999 9,n=7),and the average recovery was 99.5% (RSD=1.0% ).Conclusion The method is reproducible,accurate,precise. Adding formaldehyde can eliminate the affect of sodium metabisulfite on the determination of streptomycin sulfate.
