西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
1期
27-31
,共5页
高效液相色谱法%蒸发光散射%保健食品%果糖%葡萄糖%蔗糖%麦芽糖%乳糖%木糖
高效液相色譜法%蒸髮光散射%保健食品%果糖%葡萄糖%蔗糖%麥芽糖%乳糖%木糖
고효액상색보법%증발광산사%보건식품%과당%포도당%자당%맥아당%유당%목당
HPLC%ELSD%functional foods%fructose%glucose%sucrose%maltose%lactose%xylose
目的：建立高效液相色谱-蒸发光散射法同时测定保健食品中果糖、葡萄糖、蔗糖、麦芽糖、乳糖、木糖的方法。方法采用Sepax HP-Amino(300 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水(70∶30),流速为1.0 mL·min-1,柱温为35℃；使用蒸发光散射检测器检测,漂移管的温度105℃,载气流速2.2 mL·min-1。结果果糖、葡萄糖、蔗糖、麦芽糖、乳糖和木糖进样量分别在1.0328~10.328μg (r=0.9989),1.5085~15.085μg(r=0.9997),1.0199~10.199μg(r=0.9998),1.5094~30.187μg(r=0.9995),2.9992~59.984μg (r=0.9996),5.05~50.5μg (r=0.9996)范围内与峰面积的对数呈现良好的线性关系；平均回收率(n=6)分别为96%,105%,103%,95%,98%和99%；重复性实验,6种糖含量的 RSD(n=6)分别为2.95%,2.52%,1.75%,1.62%,2.23%和1.85%。结论该方法操作简单、快速、准确,适用于多种保健食品中糖的分析。
目的：建立高效液相色譜-蒸髮光散射法同時測定保健食品中果糖、葡萄糖、蔗糖、麥芽糖、乳糖、木糖的方法。方法採用Sepax HP-Amino(300 mm×4.6 mm,5μm)色譜柱,流動相為乙腈-水(70∶30),流速為1.0 mL·min-1,柱溫為35℃；使用蒸髮光散射檢測器檢測,漂移管的溫度105℃,載氣流速2.2 mL·min-1。結果果糖、葡萄糖、蔗糖、麥芽糖、乳糖和木糖進樣量分彆在1.0328~10.328μg (r=0.9989),1.5085~15.085μg(r=0.9997),1.0199~10.199μg(r=0.9998),1.5094~30.187μg(r=0.9995),2.9992~59.984μg (r=0.9996),5.05~50.5μg (r=0.9996)範圍內與峰麵積的對數呈現良好的線性關繫；平均迴收率(n=6)分彆為96%,105%,103%,95%,98%和99%；重複性實驗,6種糖含量的 RSD(n=6)分彆為2.95%,2.52%,1.75%,1.62%,2.23%和1.85%。結論該方法操作簡單、快速、準確,適用于多種保健食品中糖的分析。
목적：건립고효액상색보-증발광산사법동시측정보건식품중과당、포도당、자당、맥아당、유당、목당적방법。방법채용Sepax HP-Amino(300 mm×4.6 mm,5μm)색보주,류동상위을정-수(70∶30),류속위1.0 mL·min-1,주온위35℃；사용증발광산사검측기검측,표이관적온도105℃,재기류속2.2 mL·min-1。결과과당、포도당、자당、맥아당、유당화목당진양량분별재1.0328~10.328μg (r=0.9989),1.5085~15.085μg(r=0.9997),1.0199~10.199μg(r=0.9998),1.5094~30.187μg(r=0.9995),2.9992~59.984μg (r=0.9996),5.05~50.5μg (r=0.9996)범위내여봉면적적대수정현량호적선성관계；평균회수솔(n=6)분별위96%,105%,103%,95%,98%화99%；중복성실험,6충당함량적 RSD(n=6)분별위2.95%,2.52%,1.75%,1.62%,2.23%화1.85%。결론해방법조작간단、쾌속、준학,괄용우다충보건식품중당적분석。
Obj ective A method for the simultaneous determination of 6 sugars such as fructose,glucose,sucrose,maltose,lactose and xylose in functional foods was described by using high performance liquid chromatography with evaporative light scattering de-tection(ELSD).Methods Sepax HP-Amino column (300 mm×4.6 mm,5μm)was adopted,the mobile phase was composed of acetonitrile-water(70∶30)at a flow rate of 1.0 mL·min-1 ,and the column temperature was set at 35 ℃;The conditions of ELSD were as follows:the temperature of the drifting tube 105 ℃,the flow rate of nebulizer gas 2.2 mL·min-1 .Results The logarithm of samples size of fructose,glucose,sucrose,maltose,lactose and xylose had good linearity with the logarithm of peak ar-ea in the ranges of 1.032 8-10.328μg(r=0.998 9),1.508 5-15.085μg (r=0.999 7),1.019 9-10.199μg(r=0.999 8),1.509 4-30.187μg (r=0.999 5),2.999 2-59.984μg (r=0.999 6),and 5.05-50.5μg (r=0.999 6),respectively;The average recoveries were 96%,105%,103%,95%,98% and 99%,respectively;Repeatability experiments showed that RSD values of their contents were 2.95%,2.52%,1.75%,1.62%,2.23% and 1.85%,respectively.Conclusion This method was simple,fast,and accurate for the analysis of fructose,glucose,sucrose,maltose,lactose and xylose in functional foods.
