西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
1期
22-24
,共3页
余甘降脂片%没食子酸%反相高效液相色谱法%含量测定
餘甘降脂片%沒食子痠%反相高效液相色譜法%含量測定
여감강지편%몰식자산%반상고효액상색보법%함량측정
Yuganjiangzhi Tablets%gallic acid%HPLC%content determination
目的：建立高效液相色谱法测定余甘降脂片中没食子酸的含量。方法样品用体积分数50%甲醇超声提取。色谱柱为Agilent C18,流动相为甲醇-0.2 mL·L-1磷酸溶液(5∶95),流速1.0 mL·min-1,柱温25℃,检测波长273 nm。结果没食子酸在0.17~1.70μg范围内线性关系良好,平均回收率为99.9%,RSD为1.1%。结论该方法灵敏、准确、专属性强,可用于余甘降脂片的质量控制。
目的：建立高效液相色譜法測定餘甘降脂片中沒食子痠的含量。方法樣品用體積分數50%甲醇超聲提取。色譜柱為Agilent C18,流動相為甲醇-0.2 mL·L-1燐痠溶液(5∶95),流速1.0 mL·min-1,柱溫25℃,檢測波長273 nm。結果沒食子痠在0.17~1.70μg範圍內線性關繫良好,平均迴收率為99.9%,RSD為1.1%。結論該方法靈敏、準確、專屬性彊,可用于餘甘降脂片的質量控製。
목적：건립고효액상색보법측정여감강지편중몰식자산적함량。방법양품용체적분수50%갑순초성제취。색보주위Agilent C18,류동상위갑순-0.2 mL·L-1린산용액(5∶95),류속1.0 mL·min-1,주온25℃,검측파장273 nm。결과몰식자산재0.17~1.70μg범위내선성관계량호,평균회수솔위99.9%,RSD위1.1%。결론해방법령민、준학、전속성강,가용우여감강지편적질량공제。
Objective To establish an HPLC method for the determination of gallic acid in Yuganjiangzhi Tablets.Method The sam-ples were extracted by ultrasounding with 50% methanol.HPLC analysis was carried out on Agilent C18 column and the mixture of methanol-0.2 mL·L-1 phosphoric acid solution(5∶95)was used as the mobile phase,with the detection wavelength at 273 nm, and the flow rate at 1.0 mL·min-1 .Results The standard curve was linear over the range of 0.17-1.70μg,and the average re-covery was 99.9% with RSD 1.1%.Conclusion The method is sensitive,accurate and specific.It could be used for the quantita-tive determination of gallic acid in Yuganjiangzhi Tablets.
