西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
1期
14-15,16
,共3页
惠战锋%王前刚%魏谈笑%李多伟
惠戰鋒%王前剛%魏談笑%李多偉
혜전봉%왕전강%위담소%리다위
HPLC%莲子心提取物%去甲乌药碱
HPLC%蓮子心提取物%去甲烏藥堿
HPLC%련자심제취물%거갑오약감
RP-HPLC%Nelumbonucifera extract%higenamine
目的：建立测定莲子心提取物中去甲乌药碱的 HPLC 分析方法。方法色谱柱为Intersil ODS-3 C18(150 mm×4.6 mm,5μm)；流动相为乙腈-水-磷酸(450∶700∶1,其中含有4.6 g 十二烷基硫酸钠)；检测波长为284 nm；进样量为10μL；体积流量为1.0 mL·min-1；柱温30℃。结果去甲乌药碱的线性范围为32.92~164.6μg·mL-1,r=0.9999(n=5),平均回收率为98.0%,RSD为0.51%。结论该方法简单、准确、重复性好,可用于莲子心提取物中去甲乌药碱的定量测定。
目的：建立測定蓮子心提取物中去甲烏藥堿的 HPLC 分析方法。方法色譜柱為Intersil ODS-3 C18(150 mm×4.6 mm,5μm)；流動相為乙腈-水-燐痠(450∶700∶1,其中含有4.6 g 十二烷基硫痠鈉)；檢測波長為284 nm；進樣量為10μL；體積流量為1.0 mL·min-1；柱溫30℃。結果去甲烏藥堿的線性範圍為32.92~164.6μg·mL-1,r=0.9999(n=5),平均迴收率為98.0%,RSD為0.51%。結論該方法簡單、準確、重複性好,可用于蓮子心提取物中去甲烏藥堿的定量測定。
목적：건립측정련자심제취물중거갑오약감적 HPLC 분석방법。방법색보주위Intersil ODS-3 C18(150 mm×4.6 mm,5μm)；류동상위을정-수-린산(450∶700∶1,기중함유4.6 g 십이완기류산납)；검측파장위284 nm；진양량위10μL；체적류량위1.0 mL·min-1；주온30℃。결과거갑오약감적선성범위위32.92~164.6μg·mL-1,r=0.9999(n=5),평균회수솔위98.0%,RSD위0.51%。결론해방법간단、준학、중복성호,가용우련자심제취물중거갑오약감적정량측정。
Objective To establish an HPLC method for the determination of higenamine in Nelumbonucifera extract.Methods The test was carried out on an Intersil ODS-3 C18 column(150 mm×4.6 mm,5μm),with acetonitrile-water-phosphoric acid (450∶700∶1,4.6 g of lauryl sodium sulfate was dissolved in mobile phase)as the mobile phase.The detection wavelength was 284 nm, sample quantity was 10μL,and the flow rate was 1.0 mL·min-1 .The column temperature was 30 ℃.Results The calibration curves were linear in the range of 32.92-164.6 μg·mL-1 ,r=0.999 9 (n=5),and the average recovery of higenamine was 98.0%,RSD 0.51%.Conclusion The method is simple,accurate,reproducible and could be used for the determination of the content of higenamine in Nelumbo nucifera extract.
