中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2013年
1期
18-20
,共3页
肿瘤坏死因子%基因多态性%格雷夫斯病
腫瘤壞死因子%基因多態性%格雷伕斯病
종류배사인자%기인다태성%격뢰부사병
Tumor necrosis factor%Gene polymorphism%Graves,disease
目的 探讨肿瘤坏死因子-α(TNF-α)及其基因863C_A多态性与壮族甲状腺相关眼病(TAO)的相关性.方法 选择壮族TAO患者与汉族TAO患者作为研究对象,并分别选择健康壮族人与汉族人设立对照.抽取患者血液制备血清及DNA样本,分别采用酶联免疫吸附法测定血清TNF-α水平,采用聚合酶链反应(PCR)法检测TNF-α 863C_A位点基因多态性.结果 与对照组比较,TAO患者血清TNF-α水平显著升高(P＜0.05),壮族TAO患者升高更为显著(P＜0.05)；壮族人群TAO患者CA基因型频率(36.9％)显著高于汉族人群TAO患者(22.3％),AA基因型频率(19.6％)显著低于汉族人群TAO患者(21.3％)(x2=0.770、2.145,均P＜0.05).结论 TAO发病时血清TNF-α水平显著升高,在汉族与壮族之间存在差异,可能由于基因多态性引起.
目的 探討腫瘤壞死因子-α(TNF-α)及其基因863C_A多態性與壯族甲狀腺相關眼病(TAO)的相關性.方法 選擇壯族TAO患者與漢族TAO患者作為研究對象,併分彆選擇健康壯族人與漢族人設立對照.抽取患者血液製備血清及DNA樣本,分彆採用酶聯免疫吸附法測定血清TNF-α水平,採用聚閤酶鏈反應(PCR)法檢測TNF-α 863C_A位點基因多態性.結果 與對照組比較,TAO患者血清TNF-α水平顯著升高(P＜0.05),壯族TAO患者升高更為顯著(P＜0.05)；壯族人群TAO患者CA基因型頻率(36.9％)顯著高于漢族人群TAO患者(22.3％),AA基因型頻率(19.6％)顯著低于漢族人群TAO患者(21.3％)(x2=0.770、2.145,均P＜0.05).結論 TAO髮病時血清TNF-α水平顯著升高,在漢族與壯族之間存在差異,可能由于基因多態性引起.
목적 탐토종류배사인자-α(TNF-α)급기기인863C_A다태성여장족갑상선상관안병(TAO)적상관성.방법 선택장족TAO환자여한족TAO환자작위연구대상,병분별선택건강장족인여한족인설립대조.추취환자혈액제비혈청급DNA양본,분별채용매련면역흡부법측정혈청TNF-α수평,채용취합매련반응(PCR)법검측TNF-α 863C_A위점기인다태성.결과 여대조조비교,TAO환자혈청TNF-α수평현저승고(P＜0.05),장족TAO환자승고경위현저(P＜0.05)；장족인군TAO환자CA기인형빈솔(36.9％)현저고우한족인군TAO환자(22.3％),AA기인형빈솔(19.6％)현저저우한족인군TAO환자(21.3％)(x2=0.770、2.145,균P＜0.05).결론 TAO발병시혈청TNF-α수평현저승고,재한족여장족지간존재차이,가능유우기인다태성인기.
Objective To explore correlation between gene polymorphism of TNF-alpha and thyroid associated ophtalmopa thy (TAO) in Zhuang ethnic population.Methods DNA and serum samples were collected in patients with TAO in Han/Zhuang population.ELISA was applied to detect level of TNF-alpha in serum and PCR was applied to detect polymorphism of TNF-alpha gene 863C_A.Results The levels of TNF-alpha in patients with TAO increased significantly compared with control group (P ＜ 0.05),and more significantly in Zhuang population compared with Han population (P ＜ 0.05) ;gene frequency of CA was significantly higher in patients with TAO in Zhuang population than that in Han population (P ＜ 0.05),while gene frequency of AA was significantly lower (x2 =0.770,2.145,all P ＜ 0.05).Conclusion Serum level of TNF-alpha increases during occurrence of TAO,and the differences of expression level between different nationalities are due to gene polymorphism of TNF-alpha.
