中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
10期
880-882
,共3页
史长华%李玉红%胡亚涛%许倩
史長華%李玉紅%鬍亞濤%許倩
사장화%리옥홍%호아도%허천
电磁辐射%海马神经元%钙调蛋白激酶Ⅱ%环磷腺苷反应元件结合蛋白
電磁輻射%海馬神經元%鈣調蛋白激酶Ⅱ%環燐腺苷反應元件結閤蛋白
전자복사%해마신경원%개조단백격매Ⅱ%배린선감반응원건결합단백
Electromagnetic radiation%Hippocampal neuron%Calmodulin kinase Ⅱ%cAMP responsive element binding protein
目的 探讨2000 μW/cm2电磁辐射后钙调蛋白激酶Ⅱ(CaMKⅡ)和环磷腺苷反应元件结合蛋白(CREB)在元代培养海马神经元中表达的变化和意义.方法 体外培养新生大鼠大脑海马神经元,实验分为空白对照组,假辐射组和1 h/d、2 h/d、3 h/d辐射组.辐射组接受功率密度为2000 μW/cm2的近场辐射,连续辐射5d.采用Western-Blot法检测海马神经元CaMKⅡ和CREB蛋白的表达,RT-PCR法检测CaMKⅡ和CREB mRNA表达的变化.结果 电磁辐射后,1h/d、2h/d、3h/d辐射组大鼠海马神经元CaMKⅡ蛋白[分别为(0.59 ±0.05)、(0.44±0.08)、(0.18±0.04)]及其mRNA[分别为(0.41±0.08)、(0.34±0.04)、(0.24 ±0.02)]表达水平较空白对照组[(0.78±0.07)、(0.62±0.12)]明显降低(P＜0.05)；CREB蛋白[分别为(0.49±0.05)、(0.40±0.04)、(0.17 ±0.03)]及其mRNA[分别为(0.68±0.11)、(0.53±0.08)、(0.30 ±0.03)]表达水平较空白对照组[(0.69±0.10)、(0.80 ±0.12)]明显降低(P＜0.05).结论 海马神经元CaMKⅡ和CREB的表达变化可能参与了电磁辐射致大鼠学习记忆功能改变的病理生理过程.
目的 探討2000 μW/cm2電磁輻射後鈣調蛋白激酶Ⅱ(CaMKⅡ)和環燐腺苷反應元件結閤蛋白(CREB)在元代培養海馬神經元中錶達的變化和意義.方法 體外培養新生大鼠大腦海馬神經元,實驗分為空白對照組,假輻射組和1 h/d、2 h/d、3 h/d輻射組.輻射組接受功率密度為2000 μW/cm2的近場輻射,連續輻射5d.採用Western-Blot法檢測海馬神經元CaMKⅡ和CREB蛋白的錶達,RT-PCR法檢測CaMKⅡ和CREB mRNA錶達的變化.結果 電磁輻射後,1h/d、2h/d、3h/d輻射組大鼠海馬神經元CaMKⅡ蛋白[分彆為(0.59 ±0.05)、(0.44±0.08)、(0.18±0.04)]及其mRNA[分彆為(0.41±0.08)、(0.34±0.04)、(0.24 ±0.02)]錶達水平較空白對照組[(0.78±0.07)、(0.62±0.12)]明顯降低(P＜0.05)；CREB蛋白[分彆為(0.49±0.05)、(0.40±0.04)、(0.17 ±0.03)]及其mRNA[分彆為(0.68±0.11)、(0.53±0.08)、(0.30 ±0.03)]錶達水平較空白對照組[(0.69±0.10)、(0.80 ±0.12)]明顯降低(P＜0.05).結論 海馬神經元CaMKⅡ和CREB的錶達變化可能參與瞭電磁輻射緻大鼠學習記憶功能改變的病理生理過程.
목적 탐토2000 μW/cm2전자복사후개조단백격매Ⅱ(CaMKⅡ)화배린선감반응원건결합단백(CREB)재원대배양해마신경원중표체적변화화의의.방법 체외배양신생대서대뇌해마신경원,실험분위공백대조조,가복사조화1 h/d、2 h/d、3 h/d복사조.복사조접수공솔밀도위2000 μW/cm2적근장복사,련속복사5d.채용Western-Blot법검측해마신경원CaMKⅡ화CREB단백적표체,RT-PCR법검측CaMKⅡ화CREB mRNA표체적변화.결과 전자복사후,1h/d、2h/d、3h/d복사조대서해마신경원CaMKⅡ단백[분별위(0.59 ±0.05)、(0.44±0.08)、(0.18±0.04)]급기mRNA[분별위(0.41±0.08)、(0.34±0.04)、(0.24 ±0.02)]표체수평교공백대조조[(0.78±0.07)、(0.62±0.12)]명현강저(P＜0.05)；CREB단백[분별위(0.49±0.05)、(0.40±0.04)、(0.17 ±0.03)]급기mRNA[분별위(0.68±0.11)、(0.53±0.08)、(0.30 ±0.03)]표체수평교공백대조조[(0.69±0.10)、(0.80 ±0.12)]명현강저(P＜0.05).결론 해마신경원CaMKⅡ화CREB적표체변화가능삼여료전자복사치대서학습기억공능개변적병리생리과정.
Objective To investigate the changes of calmodulin kinase Ⅱ (CaMK Ⅱ) and cAMP responsive element binding protein(CREB) expressing in primary cultured hippocampal neurons and its relationship with learning and memory deficit after 2000 μW/cm2 electromagnetic radiation.Methods Primary cultured hippocampal neurons in vitro were randomly divided into normal control group,sham-radiated group,and 1 h/d,2 h/d,3 h/dradiation groups.The neurons in the radiation groups were received microwave exposure of 2000 μW/cm2.The change of CaMK Ⅱ and CREB protein in hippocampal neurons of each group of rats were measured with western blot,and the expression of CaMK Ⅱ and CREB mRNA in hippocampus were determined by RT-PCR.Results Compared with control group((0.78 ± 0.07),(0.62 ± 0.12)),the expression of CaMK Ⅱ protein (1 h/d(0.59 ±0.05),2h/d(0.44 ±0.08),3h/d(0.18 ±0.04)) and its mRNA(1h/d(0.41 ±0.08),2h/d(0.34 ±0.04),3h/d(0.24 ±0.02)) was obviously decreased (P＜0.05).Compared with control group((0.69 ±0.10),(0.80 ±0.12)),the expression of CREB protein(1h/d(0.49 ±0.05),2h/d(0.4 ±0.04),3h/d(0.17 ±0.03))and its mRNA (1 h/d (0.68 ± 0.11),2h/d (0.53 ± 0.08),3h/d (0.30 ± 0.03)) was obviously decreased after radiation(P＜0.05).Conclusion Electromagnetic radiation of 2000 μW/cm2 exposure could weaken the learning and memory abilities of rats and the decreases in the expression of CaMK Ⅱ and CREB protein and their mRNA in hippocampus may be involved in the pathophysiological process of learning and memory deficit.
