江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2014年
1期
49-52
,共4页
睡莲%幼胚%愈伤组织%培养基%植物生长调节剂%诱导
睡蓮%幼胚%愈傷組織%培養基%植物生長調節劑%誘導
수련%유배%유상조직%배양기%식물생장조절제%유도
Water lily%Young embryo%Callus%Culture medium%Plant growth regulator%Inducement
以睡莲3个杂交组合的幼胚为外植体，研究了胚龄、基本培养基、植物生长调节剂配比对愈伤组织诱导的影响。结果表明：1/2MS为最适的基本培养基；3个杂交组合在1/2MS＋2，4-D 2．0 mg/L＋6-BA 2．0 mg/L培养基中愈伤组织的平均诱导率均最高，分别为56．4％、21．5％和47．9％；授粉后13～18 d为幼胚培养的最佳时期。
以睡蓮3箇雜交組閤的幼胚為外植體，研究瞭胚齡、基本培養基、植物生長調節劑配比對愈傷組織誘導的影響。結果錶明：1/2MS為最適的基本培養基；3箇雜交組閤在1/2MS＋2，4-D 2．0 mg/L＋6-BA 2．0 mg/L培養基中愈傷組織的平均誘導率均最高，分彆為56．4％、21．5％和47．9％；授粉後13～18 d為幼胚培養的最佳時期。
이수련3개잡교조합적유배위외식체，연구료배령、기본배양기、식물생장조절제배비대유상조직유도적영향。결과표명：1/2MS위최괄적기본배양기；3개잡교조합재1/2MS＋2，4-D 2．0 mg/L＋6-BA 2．0 mg/L배양기중유상조직적평균유도솔균최고，분별위56．4％、21．5％화47．9％；수분후13～18 d위유배배양적최가시기。
Using the young embryos of 3 hybrid combinations of water lily as explants , the author studied the effects of embryo age, basic culture medium , the type and concentration of plant growth regulators on the callus inducement from the embryos .The results showed that 1/2MS was the optimum basic culture medium .The average callus inducement rates of 3 hybrid combinations in 1/2MS+2,4-D 2.0 mg/L+6-BA 2.0 mg/L were all the highest, being 56.4%, 21.5% and 47.9% respectively.When the embryo age was 13~18 days, the water lily embryo culture was easy to be successful .
