CAJ | 학술논문

以抗寒性不同的香玲和鲁果12号核桃品种为试材，在-15、-20、-25、-30、-35℃低温梯度胁迫下，分别测定了离体枝条的质膜相对透性、MDA和H2 O2含量、O2-·产生速率、SOD和CAT保护酶活性等指标，分析了低温胁迫对核桃枝条活性氧代谢的影响，结果表明：低温胁迫显著增加了核桃枝条的膜透性、O2-·产生速率、MDA和H2 O2含量，但耐寒品种鲁果12号的增幅低于香玲品种；2个品种的SOD酶活性均呈现“升-降-升-降”的趋势，CAT酶活性则先升高后降低，但不同品种之间的变化幅度较大；由此可知，鲁果12号在低温胁迫期间可有效地增加保护酶活性，抑制H2 O2的积累，从而降低活性氧对核桃的伤害，这可能是其抗寒性较强的重要原因之一。
이항한성불동적향령화로과12호핵도품충위시재，재-15、-20、-25、-30、-35℃저온제도협박하，분별측정료리체지조적질막상대투성、MDA화H2 O2함량、O2-·산생속솔、SOD화CAT보호매활성등지표，분석료저온협박대핵도지조활성양대사적영향，결과표명：저온협박현저증가료핵도지조적막투성、O2-·산생속솔、MDA화H2 O2함량，단내한품충로과12호적증폭저우향령품충；2개품충적SOD매활성균정현“승-강-승-강”적추세，CAT매활성칙선승고후강저，단불동품충지간적변화폭도교대；유차가지，로과12호재저온협박기간가유효지증가보호매활성，억제H2 O2적적루，종이강저활성양대핵도적상해，저가능시기항한성교강적중요원인지일。
Two walnut cultivars “Luguo No .12” ( most cold-resistant cultivar ) and“Xiangling” ( more cold-resistant culti-var) were taken as test materials , they were stressed and treated at -15, -20, -25, -30 and -35 ℃, the plasma membrane relative permeability, contents of MDA and H2O2, producing rate of O2 -· , and activities of protective enzymes (SOD and CAT) in their isolated braches were determined , and the effects of low temperature stress on the active oxygen metabolism of walnut bran -ches were analyzed .The results indicated that the plasma membrane permeability , producing rate of O 2 -· , and contents of MDA and H2 O2 in the branches of two walnut cultivars were significantly increased by low temperature stress , but their increment in “Lu-guo No.12” was lower than that in “Xiangling”.The SOD activity in two cultivars all presented the trend of “ascend -descend-ascend -descend”, while the CAT activity increased firstly and then decreased under low temperature stress .It was concluded that “Luguo No.12” could effectively enhance the activities of protective enzymes , further inhibit the accumulation of H 2 O2 during low temperature stress, therefore reduce the damage of active oxygen to walnut .This might be one of important reasons for higher cold-resistance of “Luguo No.12”.