CAJ | 학술논문

目的：探讨淫羊藿苷对人牙周膜细胞骨钙素(OCN)表达水平的影响，为淫羊藿苷治疗牙周病提供依据。方法：体外培养人牙周膜细胞，矿化诱导条件下将第3代细胞与10～0.001μg/mL6个浓度的淫羊藿苷作用，Elisa测定OCN表达水平。结果：人牙周膜细胞在矿化诱导条件下与不同浓度淫羊藿苷作用72小时后，1～0.01μg/mL组的OCN表达水平均较对照组显著增加(P＜0.05)；10μg/mL组OCN表达水平较对照组低，差异有统计学意义(P＜0.05)。结论：淫羊藿苷在一定浓度范围内可促进人牙周膜细胞骨向分化。
목적：탐토음양곽감대인아주막세포골개소(OCN)표체수평적영향，위음양곽감치료아주병제공의거。방법：체외배양인아주막세포，광화유도조건하장제3대세포여10～0.001μg/mL6개농도적음양곽감작용，Elisa측정OCN표체수평。결과：인아주막세포재광화유도조건하여불동농도음양곽감작용72소시후，1～0.01μg/mL조적OCN표체수평균교대조조현저증가(P＜0.05)；10μg/mL조OCN표체수평교대조조저，차이유통계학의의(P＜0.05)。결론：음양곽감재일정농도범위내가촉진인아주막세포골향분화。
Objective:To provide evidence for the application of icariin to periodontal disease by exploring the effects of icariin on the expressions of osteocalcin (OCN) in human periodontal ligament cells. Methods:Human periodontal ligament cells were cultured in vitro. Under the conditions of induced mineralization, the cells of genera-tion three were dealt with icariin in different concentrations (10~0.001μg/mL), the levels of OCN were detected by Elisa. Results:Compared with the control group, the expressions of OCN in the group of 1~0.01 μg/mL were in-creased remarkably when human periodontal ligament cells were dealt with icariin in different concentrations after 72 hours (P<0.05);the expressions of OCN in the group of 10μg/mL were less than the control group, the differ-ence had statistical meaning (P<0.05). Conclusion:icariin in a certain concentrations could promote osteogenic dif-ferentiation of human periodontal ligament cells.