CAJ | 학술논문

目的：采用HPLC法测定太行山区不同产地、不同采集时间连翘中连翘苷及连翘酯苷A的含量。方法：采用Agilent ZORBAX Eclipse XDB-C18（250mm×4.6mm，5μm）色谱柱，测定连翘苷以乙腈-水（25∶75）为流动相，等度洗脱；流速为1.0ml·min-1；检测波长为277nm。测定连翘酯苷A以乙腈-0.4％冰醋酸溶液（15∶85）为流动相，等度洗脱，流速为1.0ml·min-1；检测波长为330nm。结果：连翘苷和连翘酯苷A线性范围分别为0.206～2.060μg和0.120～1.200μg，平均回收率分别为99.52%（RSD=1.74%）和99.63%（RSD=1.21%）。结论：太行山区不同产地连翘中连翘苷和连翘酯苷A含量差异较大，7月份采集连翘中连翘苷、连翘酯苷A含量最高。含量测定方法准确、快速，适用于连翘的质量控制。
목적：채용HPLC법측정태행산구불동산지、불동채집시간련교중련교감급련교지감A적함량。방법：채용Agilent ZORBAX Eclipse XDB-C18（250mm×4.6mm，5μm）색보주，측정련교감이을정-수（25∶75）위류동상，등도세탈；류속위1.0ml·min-1；검측파장위277nm。측정련교지감A이을정-0.4％빙작산용액（15∶85）위류동상，등도세탈，류속위1.0ml·min-1；검측파장위330nm。결과：련교감화련교지감A선성범위분별위0.206～2.060μg화0.120～1.200μg，평균회수솔분별위99.52%（RSD=1.74%）화99.63%（RSD=1.21%）。결론：태행산구불동산지련교중련교감화련교지감A함량차이교대，7월빈채집련교중련교감、련교지감A함량최고。함량측정방법준학、쾌속，괄용우련교적질량공제。
Objective:To establish an HPLC method for the determination of phillyrin and Forsythia-side A in Forsythia suspense from different sites and gathering periods in Taihang mountain. Methods:Agilent ZORBAX Eclipse XDB-C18 (250mm×4.6mm,5μm) was applied. For determina-tion of phillyrin,Mobile phase was with acetonitrile-water (25∶75), the separation was carried out with a gradient program at a flow rate of 1.0ml·min-1 and detection wavelength was 277nm. For determination of Forsythoside A, Mobile phase was with acetonitrile-0.4%acetic acid Solution(15∶85),the separation was carried out with a gradient program at a flow rate of 1.0ml·min-1 and detection wavelength was 330nm. Results:Phillyrin and Forsythiaside A showed good linearity in the ranges of 0.206~2.060μg and 0.120~1.200μg with average recoveries of 99.52% and 99.63%,Respectively. Conclusions:The contents of phillyrin and Forsythiaside A in Forsythia suspensa from different sites in Taihang mountain marked difference, the contents of phillyrin and Forsythiaside A in Forsythia suspense was the highest in July,the method is simple and rapid for determination the contents of phillyrin and Forsythiaside A in Forsythia suspense,and can be used as the quality control of Forsythia suspensa.