CAJ | 학술논문

目的：探讨TIP30基因的表达对吉非替尼体外抑瘤的作用效果。方法：构建TIP30基因抑制的A549细胞，并采用靶向EGFR药物吉非替尼对TIP30基因有抑制和无抑制两组的细胞株进行处理。采用MTT法检测各组细胞生长抑制率和细胞生长情况。结果：成功构建TIP30基因抑制细胞，并在TIP30基因抑制的肺癌细胞（ TIP30＿Down ）和正常 TIP30细胞（ TIP30＿Normal ）两组均给予1μmoL/L的EGFR抑制剂吉非替尼后，TIP30＿Down细胞的生长速度明显慢于TIP30＿Normal细胞（ t＝7．21，P＜0．01）。观察5d后，TIP30＿Down组与TIP30＿Normal组肿瘤细胞抑制率分别为83．15％±3．28％和49．8％±4．63％，两组比较有统计学差异（t＝10．18，P＜0．01）。结论：TIP30基因上调可增加吉非替尼的体外抑瘤的敏感性，有望作为吉非替尼靶向治疗的预后指标。
목적：탐토TIP30기인적표체대길비체니체외억류적작용효과。방법：구건TIP30기인억제적A549세포，병채용파향EGFR약물길비체니대TIP30기인유억제화무억제량조적세포주진행처리。채용MTT법검측각조세포생장억제솔화세포생장정황。결과：성공구건TIP30기인억제세포，병재TIP30기인억제적폐암세포（ TIP30＿Down ）화정상 TIP30세포（ TIP30＿Normal ）량조균급여1μmoL/L적EGFR억제제길비체니후，TIP30＿Down세포적생장속도명현만우TIP30＿Normal세포（ t＝7．21，P＜0．01）。관찰5d후，TIP30＿Down조여TIP30＿Normal조종류세포억제솔분별위83．15％±3．28％화49．8％±4．63％，량조비교유통계학차이（t＝10．18，P＜0．01）。결론：TIP30기인상조가증가길비체니적체외억류적민감성，유망작위길비체니파향치료적예후지표。
Objective:To examine the effect of TIP 30 gene expression in the treatment with Gefitinib for lung cancer.Method:Constructed the TIP30 gene inhibited (down_regulated) A549 cells, we defined the cell line with TIP30 gene inhibited as TIP30_Down group, and the other group was TIP30_normal.We trea-ted the cell line in two groups with Gefitinib .We also detected the cell growth inhibited rate by MTT method . Results: We have successfully constructed the TIP 30 gene inhibited cell line in A 549 cells.After the two groups was treated by Gefitinib in 1μmoL/L, We found that cell growth speed of TIP 30_Down was slower than TIP30-Normal cells (t=7.21, P<0.01).After five days’ observation, the inhibition rates of cells in two groups were 83.15%±3.28%and 49.8%±4.63%, respectively.There was significant statistical difference between groups (t=10.18, P<0.01).Conclusion: TIP30 gene can increase the sensitivity of Gefitinib on the treatment of lung cancer cell line .