医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2013年
33期
96-97
,共2页
何健%黄松音%刘勇%冯慧馨
何健%黃鬆音%劉勇%馮慧馨
하건%황송음%류용%풍혜형
心肌酶%分离胶%肝素锂
心肌酶%分離膠%肝素鋰
심기매%분리효%간소리
myocardial enzymes%separation gel%Heparin lithium
目的：探讨分离胶管与肝素锂管对心肌酶谱（AST、LDH、CK、LD-1、HBDH、CK-MB）测定的影响。方法用三种试管：分离胶促凝管、肝素锂抗凝管与普通干燥管分装全血与混合血清，37℃水浴1.5与3小时后测定心肌酶谱。结果37℃水浴3小时内,全血标本随时间延长，肝素锂管组与普通干燥管组心肌酶谱中的LDH、HBDH与LD-1均有上升趋势，分离胶管组则相对比较稳定。同一时间段,混合血清分离胶管组、肝素锂管组与普通干燥管组间心肌酶谱均无显著性差异（P>0.05）。全血标本三种试管间AST、CK与CK-MB无显著性差异（P>0.05），LDH、HBDH与LD-1有极显著性差异（P<0.01）。结论分离胶管与肝素锂管的化学成份对心肌酶谱本身影响不大，但可有效延缓血细胞内心肌酶析出，比较起来，分离胶管因为隔开了血细胞与血清能更接近标本采集时的状况。
目的：探討分離膠管與肝素鋰管對心肌酶譜（AST、LDH、CK、LD-1、HBDH、CK-MB）測定的影響。方法用三種試管：分離膠促凝管、肝素鋰抗凝管與普通榦燥管分裝全血與混閤血清，37℃水浴1.5與3小時後測定心肌酶譜。結果37℃水浴3小時內,全血標本隨時間延長，肝素鋰管組與普通榦燥管組心肌酶譜中的LDH、HBDH與LD-1均有上升趨勢，分離膠管組則相對比較穩定。同一時間段,混閤血清分離膠管組、肝素鋰管組與普通榦燥管組間心肌酶譜均無顯著性差異（P>0.05）。全血標本三種試管間AST、CK與CK-MB無顯著性差異（P>0.05），LDH、HBDH與LD-1有極顯著性差異（P<0.01）。結論分離膠管與肝素鋰管的化學成份對心肌酶譜本身影響不大，但可有效延緩血細胞內心肌酶析齣，比較起來，分離膠管因為隔開瞭血細胞與血清能更接近標本採集時的狀況。
목적：탐토분리효관여간소리관대심기매보（AST、LDH、CK、LD-1、HBDH、CK-MB）측정적영향。방법용삼충시관：분리효촉응관、간소리항응관여보통간조관분장전혈여혼합혈청，37℃수욕1.5여3소시후측정심기매보。결과37℃수욕3소시내,전혈표본수시간연장，간소리관조여보통간조관조심기매보중적LDH、HBDH여LD-1균유상승추세，분리효관조칙상대비교은정。동일시간단,혼합혈청분리효관조、간소리관조여보통간조관조간심기매보균무현저성차이（P>0.05）。전혈표본삼충시관간AST、CK여CK-MB무현저성차이（P>0.05），LDH、HBDH여LD-1유겁현저성차이（P<0.01）。결론분리효관여간소리관적화학성빈대심기매보본신영향불대，단가유효연완혈세포내심기매석출，비교기래，분리효관인위격개료혈세포여혈청능경접근표본채집시적상황。
Objective: To investigate the effect on determining the concentration of myocardial enzymes (AST, LDH, CK, LD-1, HBDH, CK-MB included) between separation gel coagulation promoting tubes and heparin lithium coagulation promoting tubes. Methods: The samples of whole blood and pooled serum were respectively col ected by using separation gel coagulation promoting tubes, heparin lithium coagulation promoting tubes and traditional drying tube, then analysis the measurements of myocardial enzymes in three kinds of tubes after water bath heating in 37℃ with 1.5 and 3 hours . Results: The measurement results of LDH, HBDH and LD-1 in whole blood increased with prolong of time in both heparin lithium coagulation promoting tubes and traditional drying tube in water bath heating in 37℃ with 3 hours. However, it’s comparatively more stable in separation gel coagulation promoting tubes. There were NO significant differences (P>0.05)in the same time period among the results of determining the concentration which of myocardial enzymes in pooled serum both in three kinds of tubes neither among the results of AST, CK and CK-MB in whole blood. Nevertheless, there were highly significant differences (P<0.01)among the results of LDH, HBDH and LD-1 in whole blood on the condition mentioned above. Conclusion: Though chemical components of both separation gel and heparin lithium tube’s do not have much effect on the determining of myocardial enzymes itself, it can effectively slow down the precipitation of myocardial enzymes from cells. By comparison, separation gel tube is more accessible to the state of blood specimen col ection cause separation blood cells and serum.
