CAJ | 학술논문

目的：探讨小茴香提取物抗肝纤维化的作用及其对TGF-β/smad信号转导通路和肝星状细胞活化的影响。方法选择44只SD大鼠给予40%四氯化碳（CCl4）橄榄油溶液4 ml/（kg·体重）皮下注射5周制备肝纤维化模型，成模后随机分成治疗组和模型对照组，同时取6只正常大鼠做空白对照。治疗组给予小茴香提取物灌胃，模型对照组给予生理盐水灌胃，4周末处死大鼠检测血清ALT、AST、HA、LN，肝石蜡切片行Masson染色和抗α平滑肌动蛋白（α-SMA）、TGF-β受体Ⅰ型（TGF-βRⅠ）、TGF-β1免疫组织化学染色，反转录实时荧光定量PCR分析肝组织TGF-β1、smad2 mRNA相对内参基因甘油醛-3-磷酸脱氢酶（GAPDH）的表达量。结果小茴香提取物治疗组血清ALT、AST、HA水平，肝组织内胶原纤维含量、α-SMA、TGF-βRⅠ、TGF-β1表达以及TGF-β1、smad2 mRNA相对表达量均明显低于模型对照组。结论小茴香提取物可通过抑制TGF-β/smad信号转导通路抑制肝星状细胞活化，从而减轻大鼠肝纤维化。
목적：탐토소회향제취물항간섬유화적작용급기대TGF-β/smad신호전도통로화간성상세포활화적영향。방법선택44지SD대서급여40%사록화탄（CCl4）감람유용액4 ml/（kg·체중）피하주사5주제비간섬유화모형，성모후수궤분성치료조화모형대조조，동시취6지정상대서주공백대조。치료조급여소회향제취물관위，모형대조조급여생리염수관위，4주말처사대서검측혈청ALT、AST、HA、LN，간석사절편행Masson염색화항α평활기동단백（α-SMA）、TGF-β수체Ⅰ형（TGF-βRⅠ）、TGF-β1면역조직화학염색，반전록실시형광정량PCR분석간조직TGF-β1、smad2 mRNA상대내삼기인감유철-3-린산탈경매（GAPDH）적표체량。결과소회향제취물치료조혈청ALT、AST、HA수평，간조직내효원섬유함량、α-SMA、TGF-βRⅠ、TGF-β1표체이급TGF-β1、smad2 mRNA상대표체량균명현저우모형대조조。결론소회향제취물가통과억제TGF-β/smad신호전도통로억제간성상세포활화，종이감경대서간섬유화。
Objective To evaluate the therapeutic effects of Foeniculum Vulgare Mill in liver ifbrosis rats, and to investigate how it work on TGF-β/smad signal pathways and hepatic stellate cell activation. Methods Total of fifty male SD rats were randomly divided into 3 groups:a blank control group (BC group, 6 rats), a hepatic ifbrosis model control group (HFC group, 22 rats), a foeniculum vulgare mill treatment group (FVMT group, 22 rats). Forty-four rats of HFC group and FVMT group were given 40%CCl4 olive oil mixture by subcutaneous injection to make hepatic ifbrosis models for 5 weeks. Rats in FVMT group were given FVMT by intragastric administration, while rats in HFC group were treated with saline for weeks and then were sacriifced. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN) were detected. Histopathologic changes were observed after H.E. and Masson stainings. The expression of alpha-smooth muscle actin (α-SMA) were detected by immunohistochemical staining. TGF-β1, smad2 mRNA were detected by RT-PCR. Results The level of serum ALT, AST, HA, LN and content of collagen fibers, α-SMA, TGF-βRⅠ, TGF-β1 in the liver of FVMT group rats were significantly lower than those of HFC group. The expression of TGF-β1, smad2 mRNA were higher in HFC group than those of FVMT group. Conclusions Uygur Herb foeniculum vulgare mill may reduce liver ifbrosis by suppressing the activation of TGF-β/smad signal pathways and prevent the activation of hepatic stellate cell.