中国麻风皮肤病杂志
中國痳風皮膚病雜誌
중국마풍피부병잡지
CHINA JOURNAL OF LEPROSY AND SKIN DISEASES
2014年
4期
208-210
,共3页
足癣%红色毛癣菌%核糖体非保守区%基因序列测序
足癬%紅色毛癬菌%覈糖體非保守區%基因序列測序
족선%홍색모선균%핵당체비보수구%기인서렬측서
tinea pedis%Trichophyton rubrum%ribosomal non-conservative region%gene sequencing
目的:检测红色毛癣菌足癣复发后致病菌株基因型的变化。方法:对39例红色毛癣菌足癣复发后进行红色毛癣菌核糖体保守区(部分5.8s和ITS-2)和非转录间隔区( NTS)内的两个重复亚单位( TRS-1,TRS-2)进行PCR扩增,基因序列测定。结果:39株红色毛癣菌复发前后和标准株保守区基因序列测定比较,同源性均达99%,均为红色毛癣菌;( NTS)内的两个重复亚单位( TRS-1,TRS-2)基因序列测定比较,其中3株菌TRS-1区存在个别碱基突变,3株TRS-1区存在重复序列拷贝数变异,1株菌TRS-2区存在个别碱基突变。结论:复发后的红色毛癣菌基因型基本稳定,说明足癣复发大部分与原浅表真菌复燃有关。
目的:檢測紅色毛癬菌足癬複髮後緻病菌株基因型的變化。方法:對39例紅色毛癬菌足癬複髮後進行紅色毛癬菌覈糖體保守區(部分5.8s和ITS-2)和非轉錄間隔區( NTS)內的兩箇重複亞單位( TRS-1,TRS-2)進行PCR擴增,基因序列測定。結果:39株紅色毛癬菌複髮前後和標準株保守區基因序列測定比較,同源性均達99%,均為紅色毛癬菌;( NTS)內的兩箇重複亞單位( TRS-1,TRS-2)基因序列測定比較,其中3株菌TRS-1區存在箇彆堿基突變,3株TRS-1區存在重複序列拷貝數變異,1株菌TRS-2區存在箇彆堿基突變。結論:複髮後的紅色毛癬菌基因型基本穩定,說明足癬複髮大部分與原淺錶真菌複燃有關。
목적:검측홍색모선균족선복발후치병균주기인형적변화。방법:대39례홍색모선균족선복발후진행홍색모선균핵당체보수구(부분5.8s화ITS-2)화비전록간격구( NTS)내적량개중복아단위( TRS-1,TRS-2)진행PCR확증,기인서렬측정。결과:39주홍색모선균복발전후화표준주보수구기인서렬측정비교,동원성균체99%,균위홍색모선균;( NTS)내적량개중복아단위( TRS-1,TRS-2)기인서렬측정비교,기중3주균TRS-1구존재개별감기돌변,3주TRS-1구존재중복서렬고패수변이,1주균TRS-2구존재개별감기돌변。결론:복발후적홍색모선균기인형기본은정,설명족선복발대부분여원천표진균복연유관。
Objective:To determine the changes of DNA sequence of Trichophytin rubrum after relapse in athlete's foot. Methods:Two epetition subunits( TRS-1,TRS-2) of Trichophyton rubrum ribosomal non-con-servertive region NTS were amplified by PCR and followed by gene sequencing in 39 patients with relapsed athlete's foot. Results:The Trichophyton rubrum ribosomal non-conservative region NTS in the first and the second isolates of tinea pedis was very close ( the isogeny was 99%) . Individual base mutation happened in TRS-1 region of 3 cases or TRS-2 region of 1 case. Copy number of gene duplication sequence in TRS-1 re-gion of ribosomal non-conservative region was seen in 3 cases. Conclusion: The genotype of Trichophyton rubrum is stable in the isolates of the patients with relapsed tinea pedis. Recurrence of tinea pedis was most likely caused by the existing fungi rather than new infections with other stains of fungi.