CAJ | 학술논문

目的：明确寻常型银屑病( psoriasis vulgaris，PV)皮损组织与正常人皮肤中的差异表达蛋白。方法：收集PV皮损组织及正常人皮肤组织，提取蛋白，进行二维凝胶电泳，选择在PV皮损组织中明显差异表达的蛋白点，进行质谱和生物信息学分析。结果： PV患者角质层和真皮层二维凝胶电泳鉴别蛋白总数分别为(1961±21)和(1928±49)个，确定差异表达蛋白30个，其中14个被鉴定分型：包括角质1型细胞骨架( K1C10、K1C14、K1C15、K1C16)、角质2型细胞骨架( K2C1)，肌动蛋白( ARP 3，ACTA，ACTBM)，热休克蛋白( PHB，HSPβ1，CH60)，中心体蛋白( CP135)，膜联蛋白( ANXA4、ANXA5)。结论：差异表达蛋白可能在PV的病理形成中起重要作用，是潜在的PV诊断标记物。
목적：명학심상형은설병( psoriasis vulgaris，PV)피손조직여정상인피부중적차이표체단백。방법：수집PV피손조직급정상인피부조직，제취단백，진행이유응효전영，선택재PV피손조직중명현차이표체적단백점，진행질보화생물신식학분석。결과： PV환자각질층화진피층이유응효전영감별단백총수분별위(1961±21)화(1928±49)개，학정차이표체단백30개，기중14개피감정분형：포괄각질1형세포골가( K1C10、K1C14、K1C15、K1C16)、각질2형세포골가( K2C1)，기동단백( ARP 3，ACTA，ACTBM)，열휴극단백( PHB，HSPβ1，CH60)，중심체단백( CP135)，막련단백( ANXA4、ANXA5)。결론：차이표체단백가능재PV적병리형성중기중요작용，시잠재적PV진단표기물。
Objective:To identify the differentially expressed proteins in psoriasis vulgaris (PV) lesions and normal human skin tissue to propose potential pathological markers in psoriasis vulgaris. Methods:PV le-sions and normal human skin tissues were collected and the proteins were extracted. And then, the proteins were resolved by immobilized pH gradient-based two-dimensional electrophoresis ( 2-DE) . The differentially expressed proteins were analyzed by matrix-assisted laser desorption /ionization time of flight mass spectrome-try (MALDI-TOF-TOF-MS) and bioinformation. Results: Well-resolved 2-DE profiles of the epidermal and dermal layer were obtained,and average protein dots were (1969±21) and (1928±49) respectively;30 differentially expressed proteins were identified,14 of which were well characterized,including keratin type I,actin,heat shock protein,centrosomal protein and annexin. Conclusion:The differentially expressed proteins may play an important role in the pathogenesis of psoriasis and may be potential biomarkers in the diagnosis of PV.