CAJ | 학술논문

目的：?研究三氧化二砷（arsenic?trioxide，As2O3）对体外人肾母细胞瘤（Nephroblastoma/Wilms’?tumor，WT）SK-NEP-1细胞作用及CyclinD1表达的影响。方法?采用不同浓度的三氧化二砷作用体外培养人肾母细胞瘤WT细胞，应用MTT比色法检测细胞增殖的影响；RT-PCR法检测CyclinD1?mRNA的表达。结果?MTT检测对照组及不同药物浓度组增殖抑制率分别为（10.22±0.70）%、（20.76±3.46）%、（31.09±2.95）%、（40.04±2.30）%、（53.04±3.02）%、（64.61±0.95）%；RT-PCR法检测CyclinD1?mRNA的表达，对照组及不同药物浓度组的灰度值分别为：1.25±0.07、1.03±0.08、0.90±0.09、0.73±0.08、0.48±0.09、0.26±0.07。以上检测各组间比较差异均有统计学意义（P＜0.05）。结论?三氧化二砷可明显抑制WT细胞增殖，其机制可能与CyclinD1表达水平降低有关。
목적：?연구삼양화이신（arsenic?trioxide，As2O3）대체외인신모세포류（Nephroblastoma/Wilms’?tumor，WT）SK-NEP-1세포작용급CyclinD1표체적영향。방법?채용불동농도적삼양화이신작용체외배양인신모세포류WT세포，응용MTT비색법검측세포증식적영향；RT-PCR법검측CyclinD1?mRNA적표체。결과?MTT검측대조조급불동약물농도조증식억제솔분별위（10.22±0.70）%、（20.76±3.46）%、（31.09±2.95）%、（40.04±2.30）%、（53.04±3.02）%、（64.61±0.95）%；RT-PCR법검측CyclinD1?mRNA적표체，대조조급불동약물농도조적회도치분별위：1.25±0.07、1.03±0.08、0.90±0.09、0.73±0.08、0.48±0.09、0.26±0.07。이상검측각조간비교차이균유통계학의의（P＜0.05）。결론?삼양화이신가명현억제WT세포증식，기궤제가능여CyclinD1표체수평강저유관。
Objective?To?investigate?the?effects?of?arsenic?trioxide?on?cells?and?expressions?of?CyclinD1?in?human?Nephroblastoma?WT?cells.?Methods?WT?cells?were?exposed?to?various?concentrations?of?arsenic?trioxide?for?certain?time.?MTT?colrimetric?assay?was?performed?for?the?proliferation?inhibition?rates.?RT-PCR?was?used?to?test?the?expression?of?CyclinD1?mRNA?in?WT?cells.?ResuLts?The?proliferation?inhibition?rates?of?control?group?and?different?concentrations?of?arsenic?trioxide?groups?were?(10.22±0.70)%,(20.76±3.46)%,(31.09±2.95)%,(40.04±2.30)%,(53.04±3.02)%,(64.61±0.95)%;?To?the?expression?of?CyclinD1?mRNA,their?grey?levels?were:?1.25±0.07,1.03±0.08,0.90±0.09,0.73±0.08,0.48±0.09,0.26±0.07.To?all?the?resμLts,MμLtipe?comparison?had?signiifcant?differences(P<0.05).?Conclusions?Arsenic?trioxide?can?inhibit?WT?cells?proliferation.?Its?molecμLar?mechanisms?might?be?related?to?decrease?the?expression?of?CyclinD1?mRNA.