CAJ | 학술논문

目的：检测芍药苷对体外培养正常人表皮黑素细胞增殖活性、酪氨酸酶活性及黑素合成的影响。方法：建立正常人表皮黑素细胞培养体系，加入不同浓度(5~640μg/mL)芍药苷并作用一定时间后，采用MTT法、体外氧化DOPA反应法、NaOH法等分别测定黑素细胞增殖活性、酪氨酸酶活性及黑素含量的变化。结果：40~160μg/mL芍药苷呈剂量依赖性抑制黑素细胞增殖，与对照组比较差异有统计学意义( P<0.05)；5~20μg/mL芍药苷对酪氨酸酶活性呈剂量依赖性促进作用，与对照组比较差异有统计学意义( P<0.05)；10~20μg/mL芍药苷对黑素细胞黑素合成起促进作用，与对照组比较差异有统计学意义( P<0.05)。结论：芍药苷在较高浓度范围内(40~160μg/mL)对体外培养正常人表皮黑素细胞增殖有剂量依赖性抑制作用；在较低浓度范围内(10~20μg/mL)对其黑素合成有促进作用。
목적：검측작약감대체외배양정상인표피흑소세포증식활성、락안산매활성급흑소합성적영향。방법：건립정상인표피흑소세포배양체계，가입불동농도(5~640μg/mL)작약감병작용일정시간후，채용MTT법、체외양화DOPA반응법、NaOH법등분별측정흑소세포증식활성、락안산매활성급흑소함량적변화。결과：40~160μg/mL작약감정제량의뢰성억제흑소세포증식，여대조조비교차이유통계학의의( P<0.05)；5~20μg/mL작약감대락안산매활성정제량의뢰성촉진작용，여대조조비교차이유통계학의의( P<0.05)；10~20μg/mL작약감대흑소세포흑소합성기촉진작용，여대조조비교차이유통계학의의( P<0.05)。결론：작약감재교고농도범위내(40~160μg/mL)대체외배양정상인표피흑소세포증식유제량의뢰성억제작용；재교저농도범위내(10~20μg/mL)대기흑소합성유촉진작용。
Objective:To investigate the effect of paeoniflorin on cell proliferation,tyrosinase activity and melanin synthesis in cultured normal human melanocutes. Methods:After paeoniforin (5~640 μg/mL) was added into cultured normal human malanocytes,the effect of paeoniflorin on the proferation of melanocytes,ty-rosinase activity and synthesis of melanin in melanocytes were measured by MTT method,dopamine oxidation method and NaOH method,respectively. Results:40~160 μg/mL paeoniflorin inhibited the growth of mela-nocytes in a concentration-dependent manner ( P<0.05) . When the concentrations of 5~20μg/mL paeonifor-in were added,the tyrosinase activity was significantly increased in a concentration-dependent manner ( P<0.05). When the concentrations of 10 ~20μg/mL paeoniforin were added,the melanin synthesis were signifi-cantly increased ( P<0.05) . The differences in the results mentioned above among the test groups and control group were significant (all Ps<0.05).Conclusion: Paeoniflorin in concentrations of 40~160 μg/mL can in-hibit the proliferation in cultured normal human malanocytes in a concentration-dependent manner. When the concentration of 10~20 μg/mL is used the melanin synthesis is promoted.