CAJ | 학술논문

以蝴蝶兰品种＂V31＂为试验材料,研究了消毒时间、基本培养基、外源激素对花梗腋芽诱导的影响,以及外源激素和有机添加物对丛生芽增殖及生根壮苗的影响,结果表明：以0.1%氯化汞溶液消毒处理8 min,花梗腋芽诱导率最高,为85.19%;以1/2MS为基本培养基,花梗腋芽诱导率显著高于MS基本培养基;在1/2MS＋NAA0.5 mg/L＋椰汁100 ml/L＋蔗糖30 g/L＋琼脂7 g/L培养基上,添加6-BA 5.0 mg/L的处理,增殖系数最高,为5.53;在1/2MS＋6-BA 5.0 mg/L＋NAA 0.5 mg/L＋蔗糖30 g/L＋琼脂7 g/L培养基上,添加椰汁100 ml/L处理的增殖系数显著高于添加土豆泥、香蕉泥的处理。无根苗接种于1/2 MS＋土豆泥80 g/L＋蔗糖20 g/L＋琼脂7 g/L,添加NAA 0.5 mg/L处理的生根数和叶片数显著高于其他处理,分别为4.39条和3.03片;在1/2 MS＋NAA 0.5 mg/L＋蔗糖20 g/L＋琼脂7 g/L培养基上,添加80 g/L土豆泥的处理,生根数和叶片数显著高于添加椰汁、香蕉泥的处理。
이호접란품충＂V31＂위시험재료,연구료소독시간、기본배양기、외원격소대화경액아유도적영향,이급외원격소화유궤첨가물대총생아증식급생근장묘적영향,결과표명：이0.1%록화홍용액소독처리8 min,화경액아유도솔최고,위85.19%;이1/2MS위기본배양기,화경액아유도솔현저고우MS기본배양기;재1/2MS＋NAA0.5 mg/L＋야즙100 ml/L＋자당30 g/L＋경지7 g/L배양기상,첨가6-BA 5.0 mg/L적처리,증식계수최고,위5.53;재1/2MS＋6-BA 5.0 mg/L＋NAA 0.5 mg/L＋자당30 g/L＋경지7 g/L배양기상,첨가야즙100 ml/L처리적증식계수현저고우첨가토두니、향초니적처리。무근묘접충우1/2 MS＋토두니80 g/L＋자당20 g/L＋경지7 g/L,첨가NAA 0.5 mg/L처리적생근수화협편수현저고우기타처리,분별위4.39조화3.03편;재1/2 MS＋NAA 0.5 mg/L＋자당20 g/L＋경지7 g/L배양기상,첨가80 g/L토두니적처리,생근수화협편수현저고우첨가야즙、향초니적처리。
Phalaenopsis cultivar Dtps.Tailin Red Angel ＂V31＂ was used as tissue culture material,investigations on the effect of disinfection time,basic medium,exogenous hormone on the induction of pedicel axillary bud,and effect of exogenous hormone on the propagation,rooting ability and vigorous condition of clustered buds were carried out.The results showed that when disinfected with 0.1% HgCl2 for 8 min,the induction rate of pedicel axillary bud were highest（85.19%）.The induction rate of pedicel axillary bud were significantly higher on 1/2MS medium than MS medium.When the buds inoculated into 1/2MS＋NAA 0.5mg/L＋coconut juice 100ml/L＋sucrose 30g/L＋agar 7g/L with 5.0 mg/L 6-BA,the multiplication coefficient was highest（5.53）;Adding coconut juice 100ml/L in 1/2MS＋5.0 mg/L 6-BA＋NAA 0.5mg/L＋sucrose 30g/L＋agar 7g/L,the multiplication coefficient was significantly higher than mashed potatoes and banana slurry.When the plantlets without roots inoculated into 1/2MS ＋mashed potatoes 80g/L＋sucrose 20g/L＋ agar 7g/L with 0.5 mg/L NAA,the numbers of rooting and leaf were significantly higher than other treatments,were 4.39 and 3.03;Adding mashed potatoes 80g/L in 1/2MS＋NAA 0.5mg/L＋sucrose 20g/L＋agar 7g/L,the numbers of rooting and leaf were significantly higher than coconut juice and banana slurry.