CAJ | 학술논문

目的本实验采用色谱分离的方法从赤子爱胜蚓(Eisenia Foelide)中分离纯化出一种纯度达到95％以上的蚯蚓纤溶酶.方法采用匀浆抽提、热变性、超滤、色谱分离、SDS-PAGE和高效液相色谱法等技术对蚓激酶进行分离纯化及鉴定,并用纤维平板法测定其活性.结果通过一系列纯化步骤所得的蚓激酶比活120 000 U/mg,经SDS-PAGE分析得一条带,相对分子量为28 000 Da;高效液相色谱分析其纯度达95％以上.结论本实验从蚯蚓中分离纯化出一种高活性、高纯度的蚓激酶.
목적 본실험채용색보분리적방법종적자애성인(Eisenia Foelide)중분리순화출일충순도체도95％이상적구인섬용매.방법 채용균장추제、열변성、초려、색보분리、SDS-PAGE화고효액상색보법등기술대인격매진행분리순화급감정,병용섬유평판법측정기활성.결과 통과일계렬순화보취소득적인격매비활120 000 U/mg,경SDS-PAGE분석득일조대,상대분자량위28 000 Da;고효액상색보분석기순도체95％이상.결론 본실험종구인중분리순화출일충고활성、고순도적인격매.
Objective To obtain a lumbrokinase with a purity over 95％ from eisenia fetida earthworm by chromatography.Methods Lumbrokinase was purified through extraction,thermal denaturation,ultrafiltration,and hromategraphy from Eisenia foelide and identified by SDS-PAGE and HPLC.The activity was detected by the firbrin plate method.Results The specific activity of the obtained lumbrokinase was 120 000 U/mg.One band of 28 000Da was obtained by SDS-PAGE and HPLC showed that its purify was higher than 95％.Conclusions A single component of protein with high thrombolysis activity and high purify was separated and purified from Eisenia Foelide.