国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2013年
17期
2672-2675
,共4页
陈梅莲%付文金%陈楚填%邓任堂%李柳燕
陳梅蓮%付文金%陳楚填%鄧任堂%李柳燕
진매련%부문금%진초전%산임당%리류연
肾小球滤过率%肾小球高滤过%NGAL%KIM-1%NAG
腎小毬濾過率%腎小毬高濾過%NGAL%KIM-1%NAG
신소구려과솔%신소구고려과%NGAL%KIM-1%NAG
Glomerular filtration rate%Glomerular hyper-filtration%NGAL%KIM-1%NAG
目的 探讨肾小管损伤因子与2型糖尿病(T2DM)肾小球高滤过(GHF)的关系.方法 以基于光抑素C(CysC)的Macisaac方程式估算肾小球滤过率(eGFR),筛选T2DM患者高滤过组30例及正常滤过组58例,并设健康对照组24例.常规方法测定肌酐、CysC、血糖、糖化血红蛋白等含量,ELISA及比浊法分别检测肾小管损伤因子NGAL、KIM-1、NAG等的水平.多因素分析各指标与高滤过组eGFR的相关性.结果 高滤过组尿液NGAL、KIM-1水平均显著高于其他两组,而血浆NGAL含量却显著降低(均P＜0.05),尿液NAG水平在三组中差异无统计学意义.相关分析显示高滤过组eGFR与空腹血糖(r=0.250)、糖化血红蛋白(r=0.237)、尿液NGAL(r=0.388),KIM-1(r=0.470)等成正相关,与血浆NGAL(r=-0.159)成负相关.结论 尿液肾小管损伤因子在DM高滤过组的高水平表达,说明GHF可能直接导致肾小管早期损伤.
目的 探討腎小管損傷因子與2型糖尿病(T2DM)腎小毬高濾過(GHF)的關繫.方法 以基于光抑素C(CysC)的Macisaac方程式估算腎小毬濾過率(eGFR),篩選T2DM患者高濾過組30例及正常濾過組58例,併設健康對照組24例.常規方法測定肌酐、CysC、血糖、糖化血紅蛋白等含量,ELISA及比濁法分彆檢測腎小管損傷因子NGAL、KIM-1、NAG等的水平.多因素分析各指標與高濾過組eGFR的相關性.結果 高濾過組尿液NGAL、KIM-1水平均顯著高于其他兩組,而血漿NGAL含量卻顯著降低(均P＜0.05),尿液NAG水平在三組中差異無統計學意義.相關分析顯示高濾過組eGFR與空腹血糖(r=0.250)、糖化血紅蛋白(r=0.237)、尿液NGAL(r=0.388),KIM-1(r=0.470)等成正相關,與血漿NGAL(r=-0.159)成負相關.結論 尿液腎小管損傷因子在DM高濾過組的高水平錶達,說明GHF可能直接導緻腎小管早期損傷.
목적 탐토신소관손상인자여2형당뇨병(T2DM)신소구고려과(GHF)적관계.방법 이기우광억소C(CysC)적Macisaac방정식고산신소구려과솔(eGFR),사선T2DM환자고려과조30례급정상려과조58례,병설건강대조조24례.상규방법측정기항、CysC、혈당、당화혈홍단백등함량,ELISA급비탁법분별검측신소관손상인자NGAL、KIM-1、NAG등적수평.다인소분석각지표여고려과조eGFR적상관성.결과 고려과조뇨액NGAL、KIM-1수평균현저고우기타량조,이혈장NGAL함량각현저강저(균P＜0.05),뇨액NAG수평재삼조중차이무통계학의의.상관분석현시고려과조eGFR여공복혈당(r=0.250)、당화혈홍단백(r=0.237)、뇨액NGAL(r=0.388),KIM-1(r=0.470)등성정상관,여혈장NGAL(r=-0.159)성부상관.결론 뇨액신소관손상인자재DM고려과조적고수평표체,설명GHF가능직접도치신소관조기손상.
Objective To explore the relationship between tubular markers and glomerular hyperfiltration(GHF)in type 2 diabetes mellitus(T2DM)patients.Methods Estimated glomerular filtration rate(eGFR)was calculated by Macisaac' s formulae based on cystatin C(CysC)levels.30 cases of T2DM with GHF,58 cases of T2DM with norm-GFR and 24 healthy people were recruited in the study.Creatinine,CysC,glucose and hemoglobin A1C were measured.Tubular markers NGAL,KIM-1 and NAG were determined by ELISA or colorimetric analysis.The correlation between these markers and eGFR were conducted by multivariate analysis in the GHF group.Results The GHF group had higher urinary NGAL,KIM-1 levels and lower serum NGAL level than those in other two groups(P ＜ 0.05).There was no statistical difference in the urinary NAG level among the three groups.Correlation analysis showed that eGFR was positively related to fasting blood glucose,HbA1c,urinary NGAL,and KIM-1(r=0.250,0.237,0.388,0.470),negatively related to serum NGAL(r=-0.159)in the GHF group.Conclusion The high levels of urinary tubular damage markers are found in T2DM patients with GHF,which may be a proof that GHF can directly result in early renal tubular damage.
