CAJ | 학술논문

目的研究凋亡抑制基因XIAP与抑癌基因p53在非小细胞肺癌中的表达及两者的相关性.方法转染特异短发夹样RNA(XIAP shRNA)序列的表达载体至非小细胞肺癌细胞A549中,应用半定量PT PCR方法测定转染前后细胞中XIAP与p53的表达情况并进行对比.结果 PT-PCR结果显示XIAP在阳性转染组的表达较阴性转染组、未转染组细胞表达明显降低；p53在阳性转染组表达升高,加入顺铂后,阳性转染组XIAP mRNA表达较阴性转染组、未转染组细胞降低,p53在阳性转染加顺铂组的表达明显提高.结论非小细胞肺癌中XIAP与p53呈负相关,高表达的XIAP抑制野生型p53的表达,反之XIAP受到抑制后野生型p53表达增高,促进非小细胞肺癌细胞凋亡.
목적 연구조망억제기인XIAP여억암기인p53재비소세포폐암중적표체급량자적상관성.방법 전염특이단발협양RNA(XIAP shRNA)서렬적표체재체지비소세포폐암세포A549중,응용반정량PT PCR방법측정전염전후세포중XIAP여p53적표체정황병진행대비.결과 PT-PCR결과현시XIAP재양성전염조적표체교음성전염조、미전염조세포표체명현강저；p53재양성전염조표체승고,가입순박후,양성전염조XIAP mRNA표체교음성전염조、미전염조세포강저,p53재양성전염가순박조적표체명현제고.결론 비소세포폐암중XIAP여p53정부상관,고표체적XIAP억제야생형p53적표체,반지XIAP수도억제후야생형p53표체증고,촉진비소세포폐암세포조망.
Objective To study the expression of antiapoptosis gene XIAP and tumor suppressor gene p53 in non-small cell lung cancer as well as their respective dependencies.Methods The specific short hairpin RNA (XIAP shRNA) expression vector sequence was transfected to A549 cell.The expressions of XIAP and p53 in transfected cells was determined by semi quantitative RT-PCR.Results PT-PCR results showed that the expression of XIAP in positive transfection group was significantly decreased than that in negative transfection group and untransfected group.The expression of p53 was elevated in the positive transfection group.After the addition of cisplatin,XIAP mRNA expression in positive transfection group was lower than that in negative transfection group and untransfected group,and p53 mRNA expression increased in positive transfection plus cisplatin group.Conclusions XIAP and p53 are negatively correlated.High expression of XIAP inhibits expression of wild type p53,whereas wild-type p53 expression increases by inhibition of XIAP,which promotes apoptosis in non-small cell lung cancer.