中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
9期
1307-1309
,共3页
枯草芽孢杆菌活菌产品%皮肤过敏试验%皮肤刺激试验
枯草芽孢桿菌活菌產品%皮膚過敏試驗%皮膚刺激試驗
고초아포간균활균산품%피부과민시험%피부자격시험
Bacillus subtilis product%Skin allergy test%Skin irritation test
目的 探讨皮肤应用枯草芽孢杆菌活菌产品的安全性.方法 4只家兔背部皮肤左右分别设给药组和空白对照组,用枯草芽孢杆菌活菌产品多次局部涂抹家兔的皮肤进行刺激,观察皮肤刺激性；将豚鼠完全随机分为空白对照组、给药组和阳性对照组,每组10只,空白对照组给予赋形剂,给药组给予枯草芽孢杆菌产品,阳性对照组给予1％ 2,4-二硝基氯代苯.对皮肤多次局部涂抹致敏,观察过敏反应.结果 枯草芽孢杆菌活菌产品对家兔皮肤刺激强度为0.14分,小于0.5分,后续观察未出现可逆反应,无明显皮肤刺激性反应；给药组豚鼠致敏率为0分,致敏等级为Ⅰ级,无皮肤过敏反应.结论 枯草芽孢杆菌活菌产品局部毒性小,皮肤用药具有良好的安全性.
目的 探討皮膚應用枯草芽孢桿菌活菌產品的安全性.方法 4隻傢兔揹部皮膚左右分彆設給藥組和空白對照組,用枯草芽孢桿菌活菌產品多次跼部塗抹傢兔的皮膚進行刺激,觀察皮膚刺激性；將豚鼠完全隨機分為空白對照組、給藥組和暘性對照組,每組10隻,空白對照組給予賦形劑,給藥組給予枯草芽孢桿菌產品,暘性對照組給予1％ 2,4-二硝基氯代苯.對皮膚多次跼部塗抹緻敏,觀察過敏反應.結果 枯草芽孢桿菌活菌產品對傢兔皮膚刺激彊度為0.14分,小于0.5分,後續觀察未齣現可逆反應,無明顯皮膚刺激性反應；給藥組豚鼠緻敏率為0分,緻敏等級為Ⅰ級,無皮膚過敏反應.結論 枯草芽孢桿菌活菌產品跼部毒性小,皮膚用藥具有良好的安全性.
목적 탐토피부응용고초아포간균활균산품적안전성.방법 4지가토배부피부좌우분별설급약조화공백대조조,용고초아포간균활균산품다차국부도말가토적피부진행자격,관찰피부자격성；장돈서완전수궤분위공백대조조、급약조화양성대조조,매조10지,공백대조조급여부형제,급약조급여고초아포간균산품,양성대조조급여1％ 2,4-이초기록대분.대피부다차국부도말치민,관찰과민반응.결과 고초아포간균활균산품대가토피부자격강도위0.14분,소우0.5분,후속관찰미출현가역반응,무명현피부자격성반응；급약조돈서치민솔위0분,치민등급위Ⅰ급,무피부과민반응.결론 고초아포간균활균산품국부독성소,피부용약구유량호적안전성.
Objective To investigate and analyze the medication safety of bacillus subtilis product on skin.Methods Rabbit skin on the back of the left and right was tested in administration group and blank group respectively.The rabbit skin was irritated with bacillus subtilis living bacteria products and skin stimulus was observed;the guinea pigs were divided into the blank control group,administration group and positive control group.Blank control group was given excipients; drug group was given bacillus subtilis products and positive control group was given 1％ 2,4-nitro chlorinated benzene.Then allergic reactions of the animals were investigated.Results Skin irritation intensity of Bacillus subtilis living bacteria products on rabbit skin was 0.14 score,which was less than 0.5 score.No reversible reaction occurred andno skin irritation response occurred.Sensitization rate of dosage group was 0 grade ; the sensitization was Ⅰ grade and no skin allergy occurred.Conclusion Local toxicity of bacillus subtilis product is safe.
